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Literature summary for 3.6.5.2 extracted from
- Expression and cytoprotective activity of the small GTPase RhoB induced by the Escherichia coli cytotoxic necrotizing factor 1 (2013), Int. J. Biochem. Cell Biol., 45, 1767-1775.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cytotoxic necrotizing factor 1 | activation of Rho proteins by the cytotoxic necrotizing factor 1 (CNF1) from Escherichia coli, while the isomeric cytotoxicnecrotizing factor from Yersinia pseudotuberculosis (CNFy) drives GTP-loading of basal RhoB but fails to cause activation of the rhoB promoter and thus its expression. CNF1 inhibits cytokinesis and induces the formation of bi-nucleated (tetraploid) cells. Cytotoxic-necrotizing factors encompass a class of auto-transporter toxins produced by Escherichia coli (CNF1-3) or Yersinia pseudotuberculosis (CNFy). CNF1 specifically deamidates RhoA. CNF1-induced RhoB response depends on the deamidation of Rho proteins | Mus musculus | |
| cytotoxic necrotizing factor 1 | activation of Rho proteins by the cytotoxic necrotizing factor 1 (CNF1) from Escherichia coli, while the isomeric cytotoxicnecrotizing factor from Yersinia pseudotuberculosis (CNFy) drives GTP-loading of basal RhoB but fails tocause activation of the rhoB promoter and thus its expression. CNF1 inhibits cytokinesis and induces the formation of bi-nucleated (tetraploid) cells. Cytotoxic-necrotizing factors encompass a class of auto-transporter toxins produced by Escherichia coli (CNF1-3) or Yersiniapseudotuberculosis (CNFy). CNF1 deamidates Gln63 in RhoA. CNF1-induced RhoB response depends on the deamidation of Rho proteins | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| analysis of rhoB mRNA level by semi-quantitative real-time RT-PCR | Homo sapiens |
| analysis of rhoB mRNA level by semi-quantitative real-time RT-PCR | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P62745 | - |
- |
| Mus musculus | P62746 | - |
- |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RhoB | - |
Homo sapiens |
| RhoB | - |
Mus musculus |
| small GTPase | - |
Homo sapiens |
| small GTPase | - |
Mus musculus |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Homo sapiens | RhoB is the only member of the Rho subfamily of small GTPases, which is classified as an immediate early gene product. RhoB is up-regulated in response to growth factors as well as cytotoxic and genotoxic agents. Clostridial glucosylating toxins evoke pronounced RhoB expression, based on the inactivation of Rho/Ras proteins. Long lasting expression of RhoB in cultured cells upon activation of Rho proteins by the cytotoxic necrotizing factor 1 (CNF1) from Escherichia coli. Deamidase-deficient CNF1-C866S fails to activate the rhoB promoter, CNF1-C866S only faintly induces expression of rhoB mRNA and RhoB protein. Critical role of Rac1 in CNF1-induced RhoB expression, overview | up |
| Mus musculus | RhoB is the only member of the Rho subfamily of small GTPases, which is classified as an immediateearly gene product. RhoB is up-regulated in response to growth factors as well as cytotoxic and geno-toxic agents. Clostridial glucosylating toxins evoke pronounced RhoB expression, based on the inactivation of Rho/Ras proteins. Long lasting expression of RhoB in cultured cells upon activation of Rho proteins by the cytotoxic necrotizing factor 1 (CNF1) from Escherichia coli. CNF1-induced RhoB expression in primary and immortalized NIH3T3 fibroblasts. Deamidase-deficient CNF1-C866S fails to activate the rhoB promoter, CNF1-C866S only faintly induces expression of rhoB mRNA and RhoB protein. Critical role of Rac1 in CNF1-induced RhoB expression, overview | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | upon long term treatment with CNF1, RhoB-/- mouse embryonic fibroblasts exhibit DNA fragmentation, phosphatidylserine exposure, and loss of membrane integrity, while RhoB+/- MEFs persist as bi-nucleated (tetraploid) cells without any signs of cell death | Mus musculus |
| physiological function | a pathway involving GTPase Rac1 positively regulates the activity of the rhoB promoter and RhoB expression. Critical role of transcriptional activation in CNF1-induced RhoB expression. Regulation of the rhoB promoter, overview. RhoB dependent suppression of CNF1-induced polyploidy and cell death | Mus musculus |
| physiological function | a pathway involving GTPase Rac1 positively regulates the activity of the rhoB promoter and RhoB expression. The cytoprotective RhoB response is not only evoked by bacterial protein toxins inactivatingRho/Ras proteins but also by the Rac1-activating toxin CNF1. Critical role of transcriptional activation in CNF1-induced RhoB expression. Regulation of the rhoB promoter, overview | Homo sapiens |
| physiological function | the cytoprotective RhoB response is not only evoked by bacterial protein toxins inactivatingRho/Ras proteins but also by the Rac1-activating toxin CNF | Mus musculus |
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