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Literature summary for 3.6.5.2 extracted from
- The yeast vacuolar Rab GTPase Ypt7p has an activity beyond membrane recruitment of the homotypic fusion and protein sorting-Class C Vps complex (2012), Biochem. J., 443, 205-211.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| vacuole | - |
Saccharomyces cerevisiae | 5773 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
| Saccharomyces cerevisiae BY4742 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| vacuole preparation | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | vacuole fusion assays | Saccharomyces cerevisiae | ? | - |
? |  |
| additional information | vacuole fusion assays | Saccharomyces cerevisiae BY4742 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| vacuolar Rab GTPase | - |
Saccharomyces cerevisiae |
| Ypt7p | - |
Saccharomyces cerevisiae |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | activity of Ypt7p in proteoliposome lipid mixing: Ypt7p is required for the lipid mixing of proteoliposomes lacking cardiolipin [1,3-bis-(sn-3-phosphatidyl)-sn-glycerol]. Omission of other lipids with negatively charged and/or small head groups does not cause Ypt7p dependence for lipid mixing. Disruption of cardiolipin synthase, CRD1, does not alter dependence on Rab GTPases for vacuole fusion. Lipid mixing of proteoliposomes lacking cardiolipin is completely dependent on the presence of Ypt7p. The recruitment of the HOPS, homotypic fusion and protein sorting, complex to membranes is the main function of enzyme Ypt7p. Ypt7p therefore must stimulate membrane fusion by a mechanism that is in addition to recruitment of HOPS to the membrane. Lipid mixing required soluble NSF (N-ethylmaleimide-sensitive factor)-attachment protein receptor proteins, Sec18p and Sec17p (yeast NSF and alpha-SNAP) and the HOPS (homotypic fusion and protein sorting)-class C vacuole protein sorting complex, but not the vacuolar Rab GTPase Ypt7p | Saccharomyces cerevisiae |
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