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Literature summary for 3.6.5.2 extracted from

  • Haeusler, L.C.; Blumenstein, L.; Stege, P.; Dvorsky, R.; Ahmadian, M.R.
    Comparative functional analysis of the Rac GTPases (2003), FEBS Lett., 555, 556-560.
    View publication on PubMed
Search for more literature for 3.6.5.2

Activating Compound

Activating Compound Comment Organism Structure
Rac-specific GEF Tiam1 activates Rac3, but less efficiently than the Rac isoform Rac2 Homo sapiens
Rac-specific GEF Tiam1 stimulates the GDP exchange reaction, increases the GDP dissociation rate, activates Rac1 less efficiently than Rac2 Homo sapiens
Rac-specific GEF Tiam1 stimulates the GDP exchange reaction, increases the GDP dissociation rate, activates Rac2 more efficiently than the Rac isoforms Rac1 and Rac3 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
Rac1, expression in Escherichia coli as glutathione S-transferase fusion protein Homo sapiens
Rac2, expression in Escherichia coli as glutathione S-transferase fusion protein Homo sapiens
Rac3, expression in Escherichia coli as glutathione S-transferase fusion protein Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
p21 activated kinase PAK, inhibits nucleotide dissociation from enzyme; PAK, inhibits nucleotide dissociation from enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information nucleotide association and dissociation kinetics Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Homo sapiens Rac1 regulates gene expression, cell cycle progression and rearrangement of the actin cytoskeleton, Rac1 is ubiquitously expressed and regulates a wide variety of cellular processes GDP + phosphate
-
 ?
GTP + H2O Homo sapiens Rac2 may be responsible for the regulation of the oxidative burst in hematopoietic cells GDP + phosphate
-
 ?
GTP + H2O Homo sapiens Rac3 is ubiquitously expressed and regulates a wide variety of cellular processes GDP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Homo sapiens P60763
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Rac1 Homo sapiens
recombinant Rac2 Homo sapiens
recombinant Rac3 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
hematopoietic cell Rac2 is predominantly expressed in cells of the hematopoietic lineage Homo sapiens
-
additional information Rac1 is ubiquitously expressed Homo sapiens
-
additional information Rac3 is ubiquitously expressed Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O Rac1 regulates gene expression, cell cycle progression and rearrangement of the actin cytoskeleton, Rac1 is ubiquitously expressed and regulates a wide variety of cellular processes Homo sapiens GDP + phosphate
-
 ?
GTP + H2O Rac2 may be responsible for the regulation of the oxidative burst in hematopoietic cells Homo sapiens GDP + phosphate
-
 ?
GTP + H2O Rac3 is ubiquitously expressed and regulates a wide variety of cellular processes Homo sapiens GDP + phosphate
-
 ?
GTP + H2O nucleotide binding and hydrolysis properties, comparison with the properties of the Rac isoforms Rac1 and Rac2 Homo sapiens GDP + phosphate
-
 ?
GTP + H2O nucleotide binding and hydrolysis properties, comparison with the properties of the Rac isoforms Rac1 and Rac3, Rac2 structure, altered dynamics of Rac2 at the switch I region may be responsible for the differing biochemical properties compared with Rac1 and 3 Homo sapiens GDP + phosphate
-
 ?
GTP + H2O nucleotide binding and hydrolysis properties, comparison with the properties of the Rac isoforms Rac2 and Rac3, enzyme structure Homo sapiens GDP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
Rac1
-
 Homo sapiens
Rac2
-
 Homo sapiens
Rac3
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens