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Literature summary for 3.6.5.2 extracted from

  • Finlin, B.S.; Shao, H.; Kadono-Okuda, K.; Guo, N.; Andres, D.A.
    Rem2, a new member of the Rem/Rad/Gem/Kir family of Ras-related GTPases (2000), Biochem. J., 347, 223-231.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.5.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rattus sp.
expression in mammalian cell culture Rattus sp.

Protein Variants

Protein Variants Comment Organism
additional information truncation of C-terminal 32 residues leads to dissociation from the plasma membrane and redistribution to the cytosol Rattus sp.

General Stability

General Stability Organism
1 mM Mg2+, 0.01 mM GDP stabilize during storage Rattus sp.

Inhibitors

Inhibitors Comment Organism Structure
ethylenediaminetetraacetic acid binding of GTP completely abolished Rattus sp.
GTP competitive inhibition of GTPase activity Rattus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus sp. 5829
-
plasma membrane
-
 Rattus sp. 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 1 mM required for optimal activity, involved in binding of beta- and gamma-phosphates of GTP Rattus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30030
-
 calculation from sequence of cDNA Rattus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Rattus sp. slow time-dependent GTPase activity GDP + phosphate
-
 ir

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
near homogeneity, recombinant enzyme, affinity chromatography Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
brain mRNA expressed at highest levels Rattus sp.
-
heart barely detectable mRNA expression Rattus sp.
-
kidney mRNA expressed at highest levels Rattus sp.
-
liver low expression levels of mRNA Rattus sp.
-
lung barely detectable mRNA expression Rattus sp.
-
skeletal muscle barely detectable mRNA expression Rattus sp.
-

Storage Stability

Storage Stability Organism
-70°C, 50 mM Tris-HCl, pH 7.5, 0.01 mM GDP, 150 mM NaCl, 1 mM Mg2+, 1 mM DTT, 10% glycerol, v/v Rattus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O slow time-dependent GTPase activity Rattus sp. GDP + phosphate
-
 ir

Synonyms

Synonyms Comment Organism
Rem2 Rem/Rad/Gem/Kir family of small Ras-related GTPases Rattus sp.