Literature summary for 3.6.5.1 extracted from

Tishchenko, S.; Gabdulkhakov, A.; Tin, U.; Kostareva, O.; Lin, C.; Katanaev, V.L.
Crystallization and preliminary X-ray diffraction studies of Drosophila melanogaster Galphao-subunit of heterotrimeric G protein in complex with the RGS domain of CG5036 (2013), Acta Crystallogr. Sect. F, 69, 61-64.

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of His6-tagged truncation enzyme mutants GalphaoDELTA21 and GalphaoDELTA109 in Escherichia coli strain M15	Drosophila melanogaster

Crystallization (Commentary)

Crystallization (Comment)	Organism
Galphao-subunit of heterotrimeric G protein in complex with the RGS domain of CG5036, method screening, hanging-drop vapour-diffusion method, mixing of 0.0015 ml of 25 mg/ml protein solution containing 20 mM Tris-HCl pH 7.5, 2 mM MgCl2, 150 mM NaCl, 2 mM DTT, with 0.0015 ml of reservoir solution containing 20% PEG 4000, 100 mM HEPES sodium salt, pH 7.5, and equilibration against 0.3 ml of reservoir solution, 8-10 days, 22°C, X-ray diffraction structure determination and analysis at 2.0 A resolution, model fitting and refinement	Drosophila melanogaster

Crystallization (Comment)

Organism

Galphao-subunit of heterotrimeric G protein in complex with the RGS domain of CG5036, method screening, hanging-drop vapour-diffusion method, mixing of 0.0015 ml of 25 mg/ml protein solution containing 20 mM Tris-HCl pH 7.5, 2 mM MgCl2, 150 mM NaCl, 2 mM DTT, with 0.0015 ml of reservoir solution containing 20% PEG 4000, 100 mM HEPES sodium salt, pH 7.5, and equilibration against 0.3 ml of reservoir solution, 8-10 days, 22°C, X-ray diffraction structure determination and analysis at 2.0 A resolution, model fitting and refinement

Drosophila melanogaster

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
20000	-	x * 58000, recombinant Galphao-CG5036 complex, SDS-PAGE, x * 38000, recombinant enzyme subunit mutant GalphaoDELTA21, SDS-PAGE, x * 20000, recombinant mutant GalphaoDELTA109, SDS-PAGE	Drosophila melanogaster
38000	-	x * 58000, recombinant Galphao-CG5036 complex, SDS-PAGE, x * 38000, recombinant enzyme subunit mutant GalphaoDELTA21, SDS-PAGE, x * 20000, recombinant mutant GalphaoDELTA109, SDS-PAGE	Drosophila melanogaster
58000	-	x * 58000, recombinant Galphao-CG5036 complex, SDS-PAGE, x * 38000, recombinant enzyme subunit mutant GalphaoDELTA21, SDS-PAGE, x * 20000, recombinant mutant GalphaoDELTA109, SDS-PAGE	Drosophila melanogaster

Organism

Organism	UniProt	Comment	Textmining
Drosophila melanogaster	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His6-tagged truncation enzyme mutants GalphaoDELTA21 and GalphaoDELTA109 from Escherichia coli strain M15 by nickel affinity chromatography, for the first followed by anion exchange chromatography and gel filtration	Drosophila melanogaster

Subunits

Subunits	Comment	Organism
?	x * 58000, recombinant Galphao-CG5036 complex, SDS-PAGE, x * 38000, recombinant enzyme subunit mutant GalphaoDELTA21, SDS-PAGE, x * 20000, recombinant mutant GalphaoDELTA109, SDS-PAGE	Drosophila melanogaster

Synonyms

Synonyms	Comment	Organism
Galphao	-	Drosophila melanogaster