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Literature summary for 3.6.5.1 extracted from

  • Kamitani, S.; Ao, S.; Toshima, H.; Tachibana, T.; Hashimoto, M.; Kitadokoro, K.; Fukui-Miyazaki, A.; Abe, H.; Horiguchi, Y.
    Enzymatic actions of Pasteurella multocida toxin detected by monoclonal antibodies recognizing the deamidated alpha subunit of the heterotrimeric GTPase Gq (2011), FEBS J., 278, 2702-2712.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
Expression of the N-terminally His6-tagged mutant Galphai/q chimeric gene, in which the native N-terminus of Galphaq is replaced with that of Galphai1, the gene also posseses a TEV cleavage site, amino acids 1-28 of rat Galphai1, a linker of Arg and Ser, and the 37-359 amino acid region of mouse Galphaq, expression of recombinant wild-type Galphaq and Galphaq mutant Q209E in Mus musculus Galphaq/11-deficient embryonic fibroblasts, Swiss3T3 cells, expression of alpha subunit cDNAs of heterotrimeric GTPases, Gs, Gi-2, G13 and G11 in human 293T cells Pasteurella multocida

Protein Variants

Protein Variants Comment Organism
Q209E a Galphaq mutant, constructed for rasing of antibodies that specifically detect PMT-deaminated GTPase Galphaq Pasteurella multocida

Organism

Organism UniProt Comment Textmining
Pasteurella multocida
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-
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Synonyms

Synonyms Comment Organism
G12
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Pasteurella multocida
G13
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Pasteurella multocida
GI
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Pasteurella multocida
Gq
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Pasteurella multocida
heterotrimeric GTPase
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Pasteurella multocida

General Information

General Information Comment Organism
physiological function the virulence factor and highly potent mitogen Pasteurella multocida toxin, PMT, exhibits its toxic activity through activation of heterotrimeric GTPase-dependent pathways, by deamidating a glutamine residue in the alpha subunit of these GTPases via its C-terminal C3 domain, mechanism, overview. Galpha11 and Galphaq are substrates for PMT. C-PMT deamidates Galphaq at least tenfold more efficiently than the full-length PMT. Mutant PMT C1165S is not active on the GTPases, while the mutant C-terminal part of PMT C1159S deamidates Gai/q Pasteurella multocida