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Literature summary for 3.6.4.B8 extracted from
- Biochemical and mutational analyses of a unique clamp loader complex in the archaeon Methanosarcina acetivorans (2005), J. Biol. Chem., 280, 41852-41863.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dsDNA | the MacRFC complex (a protein complex of Mac-RFCS1, MacRFCS2, and MacRFCL) possesses very low intrinsic ATPase activity. This activity is stimulated about 12fold by dsDNA | Methanosarcina acetivorans | |
| proliferating cell nuclear antigen | the MacRFC complex (a protein complex of Mac-RFCS1, MacRFCS2, and MacRFCL) possesses very low intrinsic ATPase activity. This activity is stimulated about 3fold by Methanosarcina acetivorans proliferating cell nuclear antigen | Methanosarcina acetivorans | |
| singly primed single-stranded DNA | the MacRFC complex (a protein complex of Mac-RFCS1, MacRFCS2, and MacRFCL) possesses very low intrinsic ATPase activity. This activity is stimulated about 58fold by singly primed single-stranded DNA | Methanosarcina acetivorans | |
| ssDNA | the MacRFC complex (a protein complex of Mac-RFCS1, MacRFCS2, and MacRFCL) possesses very low intrinsic ATPase activity. This activity is stimulated about 33fold by ssDNA | Methanosarcina acetivorans |  |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | site-directed mutagenesis in the Walker A and SRC motifs is performed to examine the contribution of each subunit to the function of the Methanosarcina acetivorans clamp loader. Mutations in the large subunit MacRFCL and the small subunit MacRFCS2 do not impair clamp loading activity. Any mutant clamp loader harboring a mutation in MacRFCS1 is devoid of the clamp loading property | Methanosarcina acetivorans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
1 * 67000 (MacRFCL) + 1 * 38000 (MacRFCS2) + 1 * 35000 (MacRFCS1), SDS-PAGE of His6-tagged subunits | Methanosarcina acetivorans |
| 38000 | - |
1 * 67000 (MacRFCL) + 1 * 38000 (MacRFCS2) + 1 * 35000 (MacRFCS1), SDS-PAGE of His6-tagged subunits | Methanosarcina acetivorans |
| 67000 | - |
1 * 67000 (MacRFCL) + 1 * 38000 (MacRFCS2) + 1 * 35000 (MacRFCS1), SDS-PAGE of His6-tagged subunits | Methanosarcina acetivorans |
| 344500 | - |
gel filtration | Methanosarcina acetivorans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Methanosarcina acetivorans | - |
ADP + phosphate | - |
? | |
| ATP + H2O | Methanosarcina acetivorans DSM 2834 | - |
ADP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanosarcina acetivorans | Q8TSX5 and Q8TUC8 and Q8TPU4 | Q8TSX5: subunit MacRFCS1, Q8TUC8: subunit MacRFCS2, Q8TPU4: subunit MacRFCL | - |
| Methanosarcina acetivorans DSM 2834 | Q8TSX5 and Q8TUC8 and Q8TPU4 | Q8TSX5: subunit MacRFCS1, Q8TUC8: subunit MacRFCS2, Q8TPU4: subunit MacRFCL | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Methanosarcina acetivorans | ADP + phosphate | - |
? | |
| ATP + H2O | the clamp loader complex reconstituted from the three subunits MacRFCS1, MacRFCS2, and MacRFCL stimulates DNA synthesis by a cognate DNA polymerase in the presence of its sliding clamp. Mac-RFCS1 is critical to the clamp loading activity of the Methanosarcina acetivorans clamp loader | Methanosarcina acetivorans | ADP + phosphate | - |
? | |
| ATP + H2O | - |
Methanosarcina acetivorans DSM 2834 | ADP + phosphate | - |
? | |
| ATP + H2O | the clamp loader complex reconstituted from the three subunits MacRFCS1, MacRFCS2, and MacRFCL stimulates DNA synthesis by a cognate DNA polymerase in the presence of its sliding clamp. Mac-RFCS1 is critical to the clamp loading activity of the Methanosarcina acetivorans clamp loader | Methanosarcina acetivorans DSM 2834 | ADP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 1 * 67000 (MacRFCL) + 1 * 38000 (MacRFCS2) + 1 * 35000 (MacRFCS1), SDS-PAGE of His6-tagged subunits | Methanosarcina acetivorans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MacRFC complex | - |
Methanosarcina acetivorans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Methanosarcina acetivorans |
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