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Literature summary for 3.6.4.B10 extracted from

  • Kim, A.R.; Choi, K.W.
    TRiC/CCT chaperonins are essential for organ growth by interacting with insulin/TOR signaling in Drosophila (2019), Oncogene, 38, 4739-4754 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.4.B10

Protein Variants

Protein Variants Comment Organism
additional information gene CCT4 is knocked down by RNAi (v106099) in eye discs by using ey-Gal4 that drives GAL4 expression in eye and head primordia, all progeny die during the late pupal stage. Similar results are obtained by an additional CCT4 RNAi line (5525R-3). Dead flies in pupal cases have relatively intact thorax and abdomen but completely lack the eye-head structures. The eye-head field in larval eye discs targeted by ey-Gal4 is lost, resulting in the headless phenotype and late pupal lethality. Loss of any CCT subunit leads to similar loss-of-function phenotypes by disrupting the function of the CCT complex in vivo. Mutant phenotype, overview Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Drosophila melanogaster the CCT complex physically interacts with TOR signaling components including TOR, Rheb, and S6K ?
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Organism

Organism UniProt Comment Textmining
Drosophila melanogaster P12613 AND Q9W392 AND P48605 AND Q9VK69 AND Q7KKI0 AND Q9VXQ5 AND Q9VHL2 AND Q7K3J0 genes CCT1-8 encoding subunits CCT-alpha, CCT-beta, CCT-gamma, CCT-delta, CCT-epsilon or CCT5 isoform A, CCT-zeta, CCT-eta, and CCT-theta
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Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein InR/PI3K/Akt network in Drosophila dynamically phosphorylates sbunit CCT8 in response to insulin stimulation Drosophila melanogaster

Source Tissue

Source Tissue Comment Organism Textmining
eye
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 Drosophila melanogaster
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the CCT complex physically interacts with TOR signaling components including TOR, Rheb, and S6K Drosophila melanogaster ?
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Subunits

Subunits Comment Organism
heterohexadecamer CCT is a large double-ring complex with a central cavity. Each ring is composed of eight paralogous subunits, CCT1-CCT8. All CCT subunits are required for the CCT complex function Drosophila melanogaster

Synonyms

Synonyms Comment Organism
CCT
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 Drosophila melanogaster
CCT complex
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 Drosophila melanogaster
chaperonin containing TCP-1
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 Drosophila melanogaster

General Information

General Information Comment Organism
malfunction reduction of subunit CCT4 results in growth defects by affecting both cell size and proliferation. Loss of CCT4 causes preferential cell death anterior to the morphogenetic furrow in the eye disc and within wing pouch in the wing disc. Depletion of any CCT subunit in the eye disc results in headless phenotype and loss-of-function phenotypes by disrupting the function of the CCT complex in vivo. Overgrowth by active TOR signaling is suppressed by CCT RNAi. Loss of CCT leads to decreased phosphorylation of S6K and S6 while increasing phosphorylation of Akt. Insulin/TOR signaling is also necessary and sufficient for promoting CCT complex transcription. Reduction of the CCT complex leads to decreases in phospho-S6K and phospho-S6 while increasing phospho-Akt. CCT4 RNAi in the posterior wing disc under en-Gal4 causes early pupal lethality. Wing discs from en>CCT4 RNAi show almost complete loss of the posterior compartment marked by en>GFP. In comparison to control en>+, CCT4-depleted larvae show considerable developmental delay during its growth up to the third-instar stage, eventually resulting in pupal lethality. Consistent with the expression of ptc-Gal4 in the salivary gland, CCT4 RNAi reduced the size of the gland compared with control. In addition, knockdown of CCT4 in the wing pouch region of the developing wing disc under MS1096-Gal4 leads to loss of adult wing Drosophila melanogaster
additional information all CCT subunits are required for the CCT complex function Drosophila melanogaster
physiological function chaperonin containing TCP-1 (CCT) is a complex that assists protein folding and function. The CCT complex is required for organ growth by interacting with the TOR pathway in Drosophila melanogaster. The CCT complex regulates organ growth by directly interacting with the TOR signaling pathway, interaction with TOR signaling components including TOR, Rheb, and S6K. Analysis of the genetic interaction between CCT complex and growth signaling pathways, overview Drosophila melanogaster