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Literature summary for 3.6.4.B10 extracted from

  • Kim, S.; Lee, D.; Lee, J.; Song, H.; Kim, H.J.; Kim, K.T.
    Vaccinia-related kinase 2 controls the stability of the eukaryotic chaperonin TRiC/CCT by inhibiting the deubiquitinating enzyme USP25 (2015), Mol. Cell. Biol., 35, 1754-1762 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.4.B10

Cloned(Commentary)

Cloned (Comment) Organism
quantitative real-time reverse transcription-PCR expression analysis Mus musculus

General Stability

General Stability Organism
Vaccinia-related kinase 2 controls the stability of the eukaryotic chaperonin TRiC/CCT by inhibiting the deubiquitinating enzyme USP25 Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus P11983 AND P80314 AND P80318 AND P80315 AND P80316 AND P80317 AND P80313 AND P42932 genes CCT1-8 encoding subunits TCP-1-alpha, TCP-1-beta, TCP-1-gamma, TCP-1-delta, TCP-1-epsilon, TCP-1-zeta, TCP-1-eta, and TCP-1-theta
-

Posttranslational Modification

Posttranslational Modification Comment Organism
ubiquitination VRK2 is important for the eukaryotic chaperonin TRiC protein degradation pathway and is involved in polyQ protein aggregation. Ubiquitin-specific protease 25 (USP25) is a VRK2 substrate that acts on TRiC deubiquitination. USP25 deubiquitinates TRiC subunit CCT4. TRiC ubiquitination is induced by inhibition of USP25 and E3 ligase COP1 is recruited. Subsequently, TRiC protein levels are decreased. USP25 knockdown enhances CCT4 polyubiquitination and reduces CCT4 protein levels Mus musculus

Synonyms

Synonyms Comment Organism
chaperonin TRiC/CCT
-
 Mus musculus
TCP-1
-
 Mus musculus
TriC
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Mus musculus

General Information

General Information Comment Organism
metabolism Vaccinia-related kinase 2 controls the stability of the eukaryotic chaperonin TRiC/CCT by inhibiting the deubiquitinating enzyme USP25. VRK2 function in the negative regulation of TRiC Mus musculus
physiological function TRiC directly interacts with heat shock factor protein 1 (HSF1) and represses its transcriptional activity Mus musculus