Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.4.B10 extracted from

  • Melkani, G.C.; Bhide, S.; Han, A.; Vyas, J.; Livelo, C.; Bodmer, R.; Bernstein, S.I.
    TRiC/CCT chaperonins are essential for maintaining myofibril organization, cardiac physiological rhythm, and lifespan (2017), FEBS Lett., 591, 3447-3458 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.4.B10

Protein Variants

Protein Variants Comment Organism
additional information cardiac-specific knockdown of chaperonin CCT in Drosophila melanogaster resulting in disorganization of cardiac actin- and myosin-containing myofibrils and severe physiological dysfunction, including restricted heart diameters, elevated cardiac dysrhythmia and compromised cardiac performance. Knockdown of Cct3, Cct4, Cct5, Cct6 or Cct7 with the TinCDELTA4 driver resulted in cardiac morphological defects in one or more non-beating regions of the heart and completely non-beating hearts. These defects are not observed with TinC/+. Moreover, defects are even more severe with knockdown of Cct3, Cct4, Cct5, Cct6 or Cct7 with the Hand-Gal4 driver, whereas Hand/+ hearts do not show these defects.In addition to cardiac defects, cardiac-specific knockdown of Cct3, Cct4, Cct5, Cct6 or Cct7 has a drastic impact on the lifespan of the flies (female and male combined) compared to TinCDELTA4/+ and Hand/+ flies. Extremely severe cardiac dysfunction observed with Hand/+ upon cardiac-specific knockdown of Cct3, Cct4, Cct5, Cct6 or Cct7, correlate with further shortening of lifespan compared to TinCDELTA4/+. Quantitative analysis of additional cardiac physiological parameters, mutant phenotypes, overview Drosophila melanogaster

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Drosophila melanogaster 5737
-

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster P12613 AND Q9W392 AND P48605 AND Q9VK69 AND Q7KKI0 AND Q9VXQ5 AND Q9VHL2 AND Q7K3J0 genes CCT1-8 encoding subunits CCT-alpha, CCT-beta, CCT-gamma, CCT-delta, CCT-epsilon or CCT5 isoform A, CCT-zeta, CCT-eta, and CCT-theta
-

Source Tissue

Source Tissue Comment Organism Textmining
cardiac muscle fiber
-
 Drosophila melanogaster
-

Subunits

Subunits Comment Organism
heterohexadecamer eukaryotic cytoplasmic chaperonin TCP/TRiC/CCT forms a barrel-like structure, which is composed of 8 related subunits that are each present twice Drosophila melanogaster

Synonyms

Synonyms Comment Organism
CCT chaperonin
-
 Drosophila melanogaster
T-complex protein 1
-
 Drosophila melanogaster
TCP-1
-
 Drosophila melanogaster
TRiC/CCT
-
 Drosophila melanogaster

Expression

Organism Comment Expression
Drosophila melanogaster cardiac-specific upregulation of CCT components under time restricted feeding (TRF) up

General Information

General Information Comment Organism
malfunction cardiac-specific knockdown of CCT chaperonin significantly shortens lifespans. Additionally, disruption of circadian rhythm yields further deterioration of cardiac function of hypomorphic CCT mutants. CCT knockdown leads to disorganization of cardiac actin- and myosin-containing myofibrils and severe physiological dysfunction, including restricted heart diameters, elevated cardiac dysrhythmia and compromised cardiac performance Drosophila melanogaster
physiological function eukaryotic cytoplasmic chaperonins are key elements responsible for this proper folding of proteins, including that of cytoskeletal components. Protein folding is mediated in the eukaryotic cytosol by the TCP-1 ring large multi-subunit complex (TRiC), also called CCT. TRiC/CCT chaperonins are essential for maintaining myofibril organization, cardiac physiological rhythm, and lifespan. TRiC/CCT chaperonins are essential for actin and myosin containing myofibril integrity. Both the orchestration of protein folding and circadian rhythms mediated by CCT chaperonin are critical for maintaining heart contractility Drosophila melanogaster