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Literature summary for 3.6.4.B10 extracted from

  • Guest, S.T.; Kratche, Z.R.; Bollig-Fischer, A.; Haddad, R.; Ethier, S.P.
    Two members of the TRiC chaperonin complex, CCT2 and TCP1 are essential for survival of breast cancer cells and are linked to driving oncogenes (2015), Exp. Cell Res., 332, 223-235 .
    View publication on PubMed

Application

Application Comment Organism
medicine TRiC is a therapeutic target in breast cancer Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information RNAi based gene knockout via shRNA expressing lentivirus constructs. Analysis of overexpressed genes playing a role in mediating the growth and survival of SUM-52 breast cancer cells via large-scale RNAi-based growth and viability screen, overview. RNAi-mediated knockdown targeting CCT2 inhibits growth and colony formation of SUM-52 breast cancer cells. Knocking downTCP1 has a cell-type-specific effect on cell growth and colony forming capacity in SUM-52 cells Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P17987 AND P78371 AND P49368 AND P50991 AND P48643 AND P40227 AND Q99832 AND P50990 genes CCT1-8 encoding subunits CCT-alpha, CCT-beta, CCT-gamma, CCT-delta, CCT-epsilon, CCT-zeta-1, CCT-eta, and CCT-theta
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Source Tissue

Source Tissue Comment Organism Textmining
breast cancer cell
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Homo sapiens
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MCF-10A cell
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Homo sapiens
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SUM-52PE cell
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Homo sapiens
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Synonyms

Synonyms Comment Organism
CCT2
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Homo sapiens
TCP1
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Homo sapiens
TRiC chaperonin complex
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Homo sapiens

Expression

Organism Comment Expression
Homo sapiens role of AKT in regulating TCP1 downstream of FGFR2, Akt inhibition results in downregulation of TCP1 protein as well as reduced levels of Akt and RPS6 phosphorylation down
Homo sapiens the TCP1 subunit of TRiC is regulated by fibroblast growth factor receptor 2 (FGFR2), the induction of TCP1 by FGFR2 is inhibited by PD173074, i.e. N-[2-[[4-(diethylamino)butyl]amino-6-(3,5-dimethoxyphenyl)pyrido[2,3-d]pyrimidin-7-yl]-N'-(1,1-dimethylethyl)urea], an ATP-competitive inhibitor. FGFR2 signals through PI3K and Akt to regulate TCP1 expression up

General Information

General Information Comment Organism
malfunction RNAi-mediated knockdown targeting CCT2 inhibits growth and colony formation of SUM-52 breast cancer cells Homo sapiens
metabolism the TCP1 subunit of TRiC is regulated by FGFR2, necessary for proliferation of breast cancer cells and associated with poor overall survival of breast cancer patients. FGFR2 signals through PI3K and Akt to regulate TCP1 expression, this signaling does not require mTOR activity Homo sapiens
physiological function TRiC is a multi-protein chaperone complex that functions to assist polypeptides in achieving a functional three-dimensional configuration. It has an essential role in folding the highly abundant cytoskeletal proteins actin and tubulin The TCP1 and CCT2 genes both encode for components of a multi-protein chaperone complex in the cell known as the TCP1 containing ring complex (TRiC). The TRiC subunits TCP1 and CCT2, and potentially the entire TRiC complex, play a role in breast cancer. TCP1 and CCT2 are recurrently altered in breast cancer and necessary for growth/survival of breast cancer cells in vitro, they are determinants of overall survival in breast cancer patients. Expression of TCP1 is regulated by driver oncogene activation of PI3K signaling in breast cancer. Role for CCT2 in cell cycle progression. The TCP1 subunit of TRiC is both regulated by FGFR2 and necessary for cell growth in SUM-52 cells. the TCP1 subunit of TRiC is regulated by FGFR2, necessary for proliferation of breast cancer cells and associated with poor overall survival of breast cancer patients Homo sapiens