Literature summary for 3.6.4.B10 extracted from

Kim, J.; Shin, E.; Jeon, S.; Kim, Y.; Kim, P.; Lee, C.; Nam, S.
Overexpression, purification, and functional characterization of the group II chaperonin from the hyperthermophilic archaeum Pyrococcus horikoshii OT3 (2009), Biotechnol. Bioprocess Eng., 14, 551-558 .

No PubMed abstract available

Search for more literature for 3.6.4.B10

Cloned(Commentary)

Cloned (Comment)	Organism
the chaperonin gene PhCpn is amplified by PCR from the genomic DNA, subcloned into pET21a vector, and expressed in three Escherichia coli host cells such as BL21, Rosetta, and Codonplus (DE3)DE3. Among these host cells, Escherichia coli Rosetta shows the highest expression level of recombinant PhCpn at induction with 1 mM isopropyl beta-D-thiogalactoside	Pyrococcus horikoshii

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	with the addition of Mg2+ ion protection activity on inorganic phosphatase from thermal inactivation at 85°C and 110°C increases 2fold compared with the addition of enzyme alone	Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
59600	-	SDS-PAGE	Pyrococcus horikoshii

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus horikoshii	O57762	-	-

Purification (Commentary)

Purification (Comment)	Organism
the recombinant enzyme is purified to 91% by heat-shock treatment and anion-exchange chromatography	Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O	activity of the enzyme as a molecular chaperone is examined using hyperthermophilic inorganic phosphatase from Pyrococcus horikoshii as a model substrate. The enzyme protected the inorganic phosphatase from thermal inactivation at 85°C and 110°C	Pyrococcus horikoshii	ADP + phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 59600, SDS-PAGE	Pyrococcus horikoshii

Synonyms

Synonyms	Comment	Organism
More	ordered locus name: PH0017	Pyrococcus horikoshii
PhCPN	-	Pyrococcus horikoshii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
85	-	assay at	Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Pyrococcus horikoshii

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)