Activating Compound | Comment | Organism | Structure |
---|---|---|---|
microtubule | up to 10fold activation. Half-maximal concentration for activation 0.005 mM | Oryza sativa |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli of full-length protein and motor domain alone | Oryza sativa |
expression of the motor domain of kinesin K16 in Escherichia coli | Oryza sativa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | basal activity is independent of NaCl, microtubule-activated enzyme shows 50% inhibition at around 40 mM | Oryza sativa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microtubule | in the absence of nucleotides, 75% of K16 is associated with microtubules. In presence of ADP and ATP, 55% and 65%, respectively, are associated | Oryza sativa | 5874 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
x * 60000, calculated for full-length protein, x * 38000, SDS-PAGE and calculated for motor domain | Oryza sativa |
60000 | - |
x * 60000, calculated for full-length protein, x * 38000, SDS-PAGE and calculated for motor domain | Oryza sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Oryza sativa | - |
ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant kinesin motor domain | Oryza sativa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.62 | - |
pH 7.0, 25°C | Oryza sativa |
16.2 | - |
pH 7.0, 25°C, presence of microtubules | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Oryza sativa | ADP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, calculated for full-length protein, x * 38000, SDS-PAGE and calculated for motor domain | Oryza sativa |
Synonyms | Comment | Organism |
---|---|---|
kinesin K16 | - |
Oryza sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
basal activity | Oryza sativa |
6 | - |
microtubule-activated enzyme | Oryza sativa |