Any feedback?
Literature summary for 3.6.4.6 extracted from
- Mutant p97 exhibits species-specific changes of its ATPase activity and compromises the UBXD9-mediated monomerisation of p97 hexamers (2016), Eur. J. Cell Biol., 95, 195-207 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of GST-tagged wild-type and mutant p97 in Escherichia coli strain Arctic express RIL, coexpression with GST-tagged human UBXD9 protein in Escherichia coli in strain BL21Gold (DE3) pLysS | Homo sapiens |
| recombinant expression of GST-tagged wild-type and mutant p97 in Escherichia coli strain XL1 Blue, coexpression with GST-tagged Dictyostelium UBXD9 protein | Dictyostelium discoideum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E219K | naturally occurring mutation, constitutes variations in surface-exposed locations | Dictyostelium discoideum |
| E219K | naturally occurring mutation, the E219K mutation leads to an almost complete loss of activity | Dictyostelium discoideum |
| R154C | naturally occurring mutation, constitutes variations in surface-exposed locations, but does not alter the ATPase activity | Dictyostelium discoideum |
| R154C/E219K | naturally occurring mutation, constitutes variations in surface-exposed locations. The E219K mutation leads to an almost complete loss of activity, which is partially recuperated in the R154C/E219K double-mutant indicating p97 inter-domain communication | Dictyostelium discoideum |
| R155C | naturally occurring mutation, constitutes variations in surface-exposed locations and leads to increased ATPase activity, and a twofold decreased kd for human protein UBXD9 | Homo sapiens |
| R155H | naturally occurring mutation, constitutes variations in surface-exposed locations and leads to increased ATPase activity, no change in the kd value for human protein UBXD9 | Homo sapiens |
| R93C | naturally occurring mutation, constitutes variations in surface-exposed locations and leads to increased ATPase activity and a twofold increased kd for human protein UBXD9 | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Dictyostelium discoideum | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Homo sapiens |  |
| Mg2+ | required | Dictyostelium discoideum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Homo sapiens | - |
ADP + phosphate | - |
? | |
| ATP + H2O | Dictyostelium discoideum | - |
ADP + phosphate | - |
? | |
| additional information | Dictyostelium discoideum | UBX-domain containing cytoplasmic Dictyostelium protein UBXD9 is identified as p97 interaction partner | ? | - |
- |
|
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dictyostelium discoideum | Q75JI3 | - |
- |
| Homo sapiens | P55072 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GST-tagged wild-type and mutant p97s from Escherichia coli by glutathione affinity and cleavage of the tag by PreScission protease | Homo sapiens |
| recombinant GST-tagged wild-type and mutant p97s from Escherichia coli by glutathione affinity and cleavage of the tag by PreScission protease | Dictyostelium discoideum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Homo sapiens | ADP + phosphate | - |
? | |
| ATP + H2O | - |
Dictyostelium discoideum | ADP + phosphate | - |
? | |
| additional information | UBX-domain containing cytoplasmic Dictyostelium protein UBXD9 is identified as p97 interaction partner | Dictyostelium discoideum | ? | - |
- |
|
| additional information | UBX-domain containing human protein UBXDTUG/ASPL/UBXD9 is identified as p97 interaction partner | Homo sapiens | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homohexamer | - |
Homo sapiens |
| homohexamer | domain organization and structural information of Dictyostelium p97, overview. The N-terminal region comprising the CDC48 N and CDC48 2 domains is followed by the D1 and D2 domains and the C-terminal extension | Dictyostelium discoideum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ATPase | - |
Homo sapiens |
| ATPase | - |
Dictyostelium discoideum |
| nsfA | gen ename | Dictyostelium discoideum |
| p97 | - |
Homo sapiens |
| p97 | - |
Dictyostelium discoideum |
| triple-A ATPase | - |
Homo sapiens |
| triple-A ATPase | - |
Dictyostelium discoideum |
| VCP | - |
Homo sapiens |
| VCP | - |
Dictyostelium discoideum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Homo sapiens |
| 25 | - |
assay at | Dictyostelium discoideum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
| 7.5 | - |
assay at | Dictyostelium discoideum |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | human and Dictyostelium p97 share 81% identity and 89% similarity on the amino acid sequence level and have an almost identical order and composition of secondary structure elements | Homo sapiens |
| evolution | human and Dictyostelium p97 share 81% identity and 89% similarity on the amino acid sequence level and have an almost identical order and composition of secondary structure elements | Dictyostelium discoideum |
| malfunction | heterozygous missense mutations of p97 cause at least five human neurodegenerative disorders, i.e. R93C, R155H, and R155C mutations. All human p97 mutations lead to an increase in ATPase activity. p97 point mutations lead to differences in enzymatic activities and molecular interactions, which in the long-term result in a late-onset and progressive multisystem disease | Homo sapiens |
| malfunction | p97 point mutations lead to differences in enzymatic activities and molecularinteractions, which in the long-term result in a late-onset and progressive multisystem disease | Dictyostelium discoideum |
| additional information | UBX-domain containing Dictyostelium protein UBXD9 is identified as p97 interaction partner. Dictyostelium UBXD9 protein disassembles wild-type, but to a lesser extent mutant, p97 hexamers very efficiently into monomers. Enzyme p97 structure homology modelling using the crystal structure of mouse p97 (PDB entry 3cf2) as template | Dictyostelium discoideum |
| additional information | UBX-domain containing human protein UBXDTUG/ASPL/UBXD9 is identified as p97 interaction partner. Human UBXD9 protein disassembles wild-type, but to a lesser extent mutant, p97 hexamers very efficiently into monomers. Enzyme p97 structure homology modelling using the crystal structure of mouse p97 (PDB entry 3cf2) as template | Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
