Cloned (Comment) | Organism |
---|---|
recombinant expression of GST- and His6-tagged Vps4p and Vps4101437 in Saccharomyces cerevisiae strain BY4741 | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a Vps4p deletion mutant | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Saccharomyces cerevisiae | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharomyces cerevisiae | the tombusvirus p33 replication protein interacts with Vps4p and three ESCRT-III proteins | ? | - |
? | |
additional information | Saccharomyces cerevisiae BY4741 | the tombusvirus p33 replication protein interacts with Vps4p and three ESCRT-III proteins | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae BY4741 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST- and His6-tagged Vps4p and Vps4101437 from Saccharomyces cerevisiae strain BY4741 | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the tombusvirus p33 replication protein interacts with Vps4p and three ESCRT-III proteins | Saccharomyces cerevisiae | ? | - |
? | |
additional information | the tombusvirus p33 replication protein interacts with Vps4p and three ESCRT-III proteins | Saccharomyces cerevisiae BY4741 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Vps4p | - |
Saccharomyces cerevisiae |
Vps4p AAA ATPase | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
malfunction | deletion of Vps4p in yeast leads to the formation of crescent-like membrane structures instead of the characteristic spherule and vesicle-like structures | Saccharomyces cerevisiae |
physiological function | Vps4p is required for the formation of spherule-like structures induced by tombusvirus replication proteins in yeast. Key role for the conserved Vps4p AAA ATPase, whose canonical function is to disassemble the ESCRT complexes, i.e. endosomal sorting complexes required for transport, and recycle them from the membranes back to the cytosol. Vps4p is recruited to become a permanent component of the viral replicase complexes, it is co-localized with the viral dsRNA and contacts the viral (+)RNA in the intracellular membrane | Saccharomyces cerevisiae |