Cloned (Comment) | Organism |
---|---|
gene cshA, recombinant expression of FLAG-Strep-tagged wild-type and mutant enzymes in Escherichia coli strain Rosetta (DE3) | Staphylococcus aureus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Staphylococcus aureus | - |
ADP + phosphate | - |
? | |
ATP + H2O | Staphylococcus aureus NCTC 8325 | - |
ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | Q2FWH5 | - |
- |
Staphylococcus aureus NCTC 8325 | Q2FWH5 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant FLAG-Strep-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta (DE3) by two steps of affinity chromatography, recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Staphylococcus aureus | ADP + phosphate | - |
? | |
ATP + H2O | - |
Staphylococcus aureus NCTC 8325 | ADP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CshA | - |
Staphylococcus aureus |
DEAD-box RNA helicase | - |
Staphylococcus aureus |
RNA helicase CshA | - |
Staphylococcus aureus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at, ATPase assay | Staphylococcus aureus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at, ATPase assay | Staphylococcus aureus |
General Information | Comment | Organism |
---|---|---|
malfunction | a selected subset of RNAs is significantly stabilised in absence of the RNA helicase | Staphylococcus aureus |
additional information | the C-terminal region of the RNA helicase CshA is required for the interaction with the degradosome and turnover of bulk RNA in the opportunistic pathogen Staphylococcus aureus. Half-lives of diverse RNAs in the wild-type and mutant CshA strains, correlation of half life and steady-state levels, detailed overview | Staphylococcus aureus |
physiological function | CshA is required for efficient turnover of the bulk of mRNAs. For efficient degradation, the RNA helicase interacts through its C-terminal extension with the degradosome components. The molecular function of the RNA helicase might be the destabilisation of secondary structures or the removal of hindering RNA binding proteins | Staphylococcus aureus |