Cloned (Comment) | Organism |
---|---|
expression of NS3 as maltose-binding protein furion protein using the constitutive elongation factor-1alpha promoter in HEK-293T cells. Plant viral RSS protein NS3 complements HIV-1 Tat based on the sequestration of small dsRNA | Rice hoja blanca tenuivirus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | an NS3 mutant, that is deficient in RNA binding and its associated RSS activity, is inactive in complementing the RNA silencing suppressor function of the Tat protein of Human immunodeficiency virus type 1 | Rice hoja blanca tenuivirus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
x * 66000, recombinant NS3, SDS-PAGE, x * 109000, recombinant MBP-fusion NS3 protein, SDS-PAGE | Rice hoja blanca tenuivirus |
109000 | - |
x * 66000, recombinant NS3, SDS-PAGE, x * 109000, recombinant MBP-fusion NS3 protein, SDS-PAGE | Rice hoja blanca tenuivirus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rice hoja blanca tenuivirus | the NS3 protein of Rice hoja blanca virus is an RNA silencing suppressor, RSS, that exclusively binds to small dsRNA molecules. This plant viral RSS lacks interferon antagonistic activity, yet it is able to substitute the RSS function of the Tat protein of Human immunodeficiency virus type 1 based on the sequestration of small dsRNA. NS3 is able to inhibit endogenous miRNA action in mammalian cells | ? | - |
? | |
additional information | Rice hoja blanca tenuivirus RHBV | the NS3 protein of Rice hoja blanca virus is an RNA silencing suppressor, RSS, that exclusively binds to small dsRNA molecules. This plant viral RSS lacks interferon antagonistic activity, yet it is able to substitute the RSS function of the Tat protein of Human immunodeficiency virus type 1 based on the sequestration of small dsRNA. NS3 is able to inhibit endogenous miRNA action in mammalian cells | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rice hoja blanca tenuivirus | Q67897 | RHBV | - |
Rice hoja blanca tenuivirus RHBV | Q67897 | RHBV | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the NS3 protein of Rice hoja blanca virus is an RNA silencing suppressor, RSS, that exclusively binds to small dsRNA molecules. This plant viral RSS lacks interferon antagonistic activity, yet it is able to substitute the RSS function of the Tat protein of Human immunodeficiency virus type 1 based on the sequestration of small dsRNA. NS3 is able to inhibit endogenous miRNA action in mammalian cells | Rice hoja blanca tenuivirus | ? | - |
? | |
additional information | the NS3 protein of Rice hoja blanca virus is an RNA silencing suppressor, RSS, that exclusively binds to small dsRNA molecules. This plant viral RSS lacks interferon antagonistic activity, yet it is able to substitute the RSS function of the Tat protein of Human immunodeficiency virus type 1 based on the sequestration of small dsRNA. NS3 is able to inhibit endogenous miRNA action in mammalian cells | Rice hoja blanca tenuivirus RHBV | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 66000, recombinant NS3, SDS-PAGE, x * 109000, recombinant MBP-fusion NS3 protein, SDS-PAGE | Rice hoja blanca tenuivirus |
Synonyms | Comment | Organism |
---|---|---|
non-structural protein 3 protein | - |
Rice hoja blanca tenuivirus |
NS3 protein | - |
Rice hoja blanca tenuivirus |