Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3), recombinant protein, NS3d2wt variant corresponding to wild-type domain 2, NS3d2D construct comprises the complete domain, HCV(1361-1503) without loop, pET21b andpET16b vectors | Hepacivirus C |
expression of the Arg-rich amino acid motif HCV1487-1500, of the complete domain 2, and of domain 2 lacking the flexible loop localized between Val1458 and Thr1476 as His10-tagged proteins in Escherichia coli strain BL21(DE3) | Hepacivirus C |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | domain 2 of wild-type NS3 protein and domain 2 devoid of the loop structure used for inhibition studies on functions of protein kinase C (PKC), inhibitory potential towards the majority of protein kinase C isoforms shown | Hepacivirus C |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Hepacivirus C | the Arg-rich amino acid motif HCV1487-1500, a fragment of domain 2 NS3 of Hepatitis C virus, as well as the complete domain 2, and domain 2 lacking the flexible loop localized between Val1458 and Thr1476, mediate competitive inhibition of diverse protein kinase C functions, inhibition of rat brain PKC, overview | ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hepacivirus C | P27958 | - |
- |
Purification (Comment) | Organism |
---|---|
gel filtration, SDS-PAGE | Hepacivirus C |
recombinant His10-tagged Arg-rich amino acid motif HCV1487-1500, complete domain 2, and domain 2 lacking the flexible loop from Escherichia coli strain BL21(DE3) | Hepacivirus C |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
surface of domain 2 of the NS3 NTPase/helicase in direct vicinity to a flexible loop that is localized between Val1458 and Thr1476, accessibility of the Arg-rich amino acid motif by this loop for protein kinase C inhibition analyzed, two variants of domain 2 generated, in vitro protein kinase C (PKC) phosphorylation studies, binding and competition assays, modelling of ribbon diagrams, presence of the intact loop abolishes the binding of domain 2 to a tailed duplex RNA, binding of dsDNA not affected, loop structure reduces the extent of inhibition of protein kinase C (PKC) by domain 2 and regulates the binding of dsRNA, various mechanisms by which the NS3 protein perturb signal transduction in infected cells | Hepacivirus C |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Hepacivirus C | ADP + phosphate | - |
? | |
ATP + H2O | the Arg-rich amino acid motif HCV1487-1500, a fragment of domain 2 NS3 of Hepatitis C virus, as well as the complete domain 2, and domain 2 lacking the flexible loop localized between Val1458 and Thr1476, mediate competitive inhibition of diverse protein kinase C functions, inhibition of rat brain PKC, overview | Hepacivirus C | ADP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
nonstructural protein 3 | - |
Hepacivirus C |
nonstructural protein 3 | ambiguous | Hepacivirus C |
NS3 | - |
Hepacivirus C |
NS3 protein | ambiguous | Hepacivirus C |
NTPase/helicase | - |
Hepacivirus C |