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Literature summary for 3.6.4.13 extracted from

  • Xu, T.; Sampath, A.; Chao, A.; Wen, D.; Nanao, M.; Chene, P.; Vasudevan Subhash, G.; Lescar, J.
    Structure of the Dengue virus helicase/nucleoside triphosphatase catalytic domain at a resolution of 2.4 A (2005), J. Virol., 79, 10278-10288.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
drug development the enzyme is a target for development of specific antiviral inhibitors Dengue virus

Crystallization (Commentary)

Crystallization (Comment) Organism
purified catalytic domain fragment, hanging drop vapour diffusion method, 0.002 ml of 10 mg/ml protein in 0.1 M MES, pH 6.5, 0.2 M ammonium sulfate, 14% PEG 8000, mixed with an equal volume of precipitation solution, 18°C, macroseeding, cryoprotection by 25% glycerol, X-ray diffraction structure determination and analysis at 2.4 A resolution, modeling Dengue virus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ metal-dependent NTPase activity, mechanism involves a bound sulfate ion Dengue virus
sulfate bound, required for the metal-dependent NTPase reaction mechanism Dengue virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Dengue virus RNA helicase activity ADP + phosphate
-
?
additional information Dengue virus the enzyme plays an important role in viral replication ?
-
?

Organism

Organism UniProt Comment Textmining
Dengue virus
-
i.e. DNV
-

Reaction

Reaction Comment Organism Reaction ID
a nucleoside triphosphate + H2O = a nucleoside diphosphate + phosphate catalytic mechanism involving a bound sulfate ion, NTPase active site structure, nucleic acid binding site Dengue virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O RNA helicase activity Dengue virus ADP + phosphate
-
?
additional information the enzyme plays an important role in viral replication Dengue virus ?
-
?
additional information multifunctional enzyme showing protease, helicase, and NTPase activities Dengue virus ?
-
?

Subunits

Subunits Comment Organism
dimer crystal structure, three-domain structure with asymmetric distribution of charges on the surface and a tunnel structure for RNA substrate access, overview Dengue virus

Synonyms

Synonyms Comment Organism
helicase/nucleoside triphosphatase
-
Dengue virus
NS3
-
Dengue virus
nucleoside 5'-triphosphatase
-
Dengue virus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Dengue virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Dengue virus