Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.4.10 extracted from

  • Fernandez-Higuero, J.A.; Aguado, A.; Perales-Calvo, J.; Moro, F.; Muga, A.
    Activation of the DnaK-ClpB complex is regulated by the properties of the bound substrate (2018), Sci. Rep., 8, 5796 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.4.10

Activating Compound

Activating Compound Comment Organism Structure
aggregated alpha-casein
-
 Escherichia coli
aggregated glucose-6-phosphate dehydrogenase
-
 Escherichia coli
aggregated RepE
-
 Escherichia coli
additional information aggregates of carboxymethylated alpha-lactalbumin, fluorescein-CALLQSRLLLSAPRRAAATAR, NRLLLTG, and tryptic fragments of casein smaller than 10 kDa fail to stimulate the DnaK-ClpB complex activity Escherichia coli

Protein Variants

Protein Variants Comment Organism
D526A the DnaK mutant is unable to render a stimulated bichaperone complex with ClpB and doe s not reactivate glucose-6-phosphate dehydrogenase aggregates Escherichia coli
E274A/E678A inactive ClpB mutant Escherichia coli
K70A the DnaK mutant shows impaired ATP hydrolysis activity Escherichia coli
T199A the DnaK mutant shows impaired ATP hydrolysis activity Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 20 mM used in assay conditions Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Escherichia coli
-
 ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Escherichia coli ADP + phosphate
-
 ?
ATP + H2O + aggregated alpha-casein
-
 Escherichia coli ADP + phosphate + activated alpha-casein
-
 ?
ATP + H2O + aggregated glucose-6-phosphate dehydrogenase
-
 Escherichia coli ADP + phosphate + activated glucose-6-phosphate dehydrogenase
-
 ?
ATP + H2O + aggregated RepE
-
 Escherichia coli ADP + phosphate + activated RepE
-
 ?
additional information functional coupling of both chaperones ClpB and DnaK depends on specific properties of the client protein they interact with Escherichia coli ?
-
-

Synonyms

Synonyms Comment Organism
ClpB
-
 Escherichia coli
disaggregase
-
 Escherichia coli
DnaK
-
 Escherichia coli
Hsp70
-
 Escherichia coli

General Information

General Information Comment Organism
metabolism ClpB interacts differently with DnaK in the presence of aggregates or small peptides, displaying a higher affinity for aggregate-bound DnaK. DnaK-ClpB collaboration requires the coupled ATPase-dependent remodeling activities of both chaperones Escherichia coli