Activating Compound | Comment | Organism | Structure |
---|---|---|---|
aggregated alpha-casein | - |
Escherichia coli | |
aggregated glucose-6-phosphate dehydrogenase | - |
Escherichia coli | |
aggregated RepE | - |
Escherichia coli | |
additional information | aggregates of carboxymethylated alpha-lactalbumin, fluorescein-CALLQSRLLLSAPRRAAATAR, NRLLLTG, and tryptic fragments of casein smaller than 10 kDa fail to stimulate the DnaK-ClpB complex activity | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D526A | the DnaK mutant is unable to render a stimulated bichaperone complex with ClpB and doe s not reactivate glucose-6-phosphate dehydrogenase aggregates | Escherichia coli |
E274A/E678A | inactive ClpB mutant | Escherichia coli |
K70A | the DnaK mutant shows impaired ATP hydrolysis activity | Escherichia coli |
T199A | the DnaK mutant shows impaired ATP hydrolysis activity | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 20 mM used in assay conditions | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Escherichia coli | - |
ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Escherichia coli | ADP + phosphate | - |
? | |
ATP + H2O + aggregated alpha-casein | - |
Escherichia coli | ADP + phosphate + activated alpha-casein | - |
? | |
ATP + H2O + aggregated glucose-6-phosphate dehydrogenase | - |
Escherichia coli | ADP + phosphate + activated glucose-6-phosphate dehydrogenase | - |
? | |
ATP + H2O + aggregated RepE | - |
Escherichia coli | ADP + phosphate + activated RepE | - |
? | |
additional information | functional coupling of both chaperones ClpB and DnaK depends on specific properties of the client protein they interact with | Escherichia coli | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
ClpB | - |
Escherichia coli |
disaggregase | - |
Escherichia coli |
DnaK | - |
Escherichia coli |
Hsp70 | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
metabolism | ClpB interacts differently with DnaK in the presence of aggregates or small peptides, displaying a higher affinity for aggregate-bound DnaK. DnaK-ClpB collaboration requires the coupled ATPase-dependent remodeling activities of both chaperones | Escherichia coli |