Activating Compound | Comment | Organism | Structure |
---|---|---|---|
kappa-casein | the enzyme exhibits a weak ATPase activity that is stimulated about 2fold by kappa-casein | Leptospira interrogans | |
kappa-casein | the enzyme exhibits a weak ATPase activity that is stimulated about 4fold by kappa-casein | Escherichia coli | |
additional information | aggregated glucose-6-phosphate dehydrogenase does not significantly affect the ATPase activity of the enzyme | Escherichia coli | |
additional information | aggregated glucose-6-phosphate dehydrogenase does not significantly affect the ATPase activity of the enzyme | Leptospira interrogans | |
poly-L-lysine | the enzyme exhibits a weak ATPase activity that is stimulated about 10fold by poly-L-lysine | Escherichia coli | |
poly-L-lysine | the enzyme exhibits a weak ATPase activity that is stimulated about 5fold by poly-L-lysine | Leptospira interrogans |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
expressed in Escherichia coli BL21(DE3) cells | Leptospira interrogans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 10 mM used in assay conditions | Escherichia coli | |
Mg2+ | 10 mM used in assay conditions | Leptospira interrogans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Escherichia coli | - |
ADP + phosphate | - |
? | |
ATP + H2O | Leptospira interrogans | - |
ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Leptospira interrogans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Co-NTA agarose column chromatography and Superdex S200 gel filtration | Escherichia coli |
Co-NTA agarose column chromatography and Superdex S200 gel filtration | Leptospira interrogans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Escherichia coli | ADP + phosphate | - |
? | |
ATP + H2O | - |
Leptospira interrogans | ADP + phosphate | - |
? | |
ATP + H2O + aggregated fructose-1,6-bisphosphate aldolase | - |
Escherichia coli | ADP + phosphate + activated fructose-1,6-bisphosphate aldolase | - |
? | |
ATP + H2O + aggregated fructose-1,6-bisphosphate aldolase | - |
Leptospira interrogans | ADP + phosphate + activated fructose-1,6-bisphosphate aldolase | - |
? | |
ATP + H2O + aggregated glucose-6-phosphate dehydrogenase | - |
Escherichia coli | ADP + phosphate + activated glucose-6-phosphate dehydrogenase | - |
? | |
ATP + H2O + aggregated glucose-6-phosphate dehydrogenase | - |
Leptospira interrogans | ADP + phosphate + activated glucose-6-phosphate dehydrogenase | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 100000, SDS-PAGE | Leptospira interrogans |
Synonyms | Comment | Organism |
---|---|---|
AAA+ chaperone | - |
Escherichia coli |
AAA+ chaperone | - |
Leptospira interrogans |
ClpB | - |
Escherichia coli |
ClpB | - |
Leptospira interrogans |
molecular chaperone | - |
Escherichia coli |
molecular chaperone | - |
Leptospira interrogans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
67 | - |
melting temperature | Leptospira interrogans |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme's importance during infection is due to its role as a molecular chaperone involved in reactivation of protein aggregates | Escherichia coli |
physiological function | the enzyme's importance during infection is due to its role as a molecular chaperone involved in reactivation of protein aggregates | Leptospira interrogans |