Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Leptospira interrogans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Leptospira interrogans | - |
ADP + phosphate | - |
? | |
ATP + H2O | Leptospira interrogans B42 | - |
ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leptospira interrogans | - |
serovar Copenhageni | - |
Leptospira interrogans B42 | - |
serovar Copenhageni | - |
Purification (Comment) | Organism |
---|---|
HisPur cobalt resin column chromatography and Superdex S200 gel filtration | Leptospira interrogans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Leptospira interrogans | ADP + phosphate | - |
? | |
ATP + H2O | - |
Leptospira interrogans B42 | ADP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 100000, His6-tagged enzymeSDS-PAGE | Leptospira interrogans |
? | x * 98500, His6-tagged enzyme, calculated from amino acid sequence | Leptospira interrogans |
Synonyms | Comment | Organism |
---|---|---|
ClpB | - |
Leptospira interrogans |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme reactivates aggregated proteins in cooperation with the DnaK chaperone system and promotes survival of bacteria under stress conditions. The enzyme can support the virulence of Leptospira interrogans by protecting the conformational integrity and catalytic activity of multiple metabolic enzymes, thus maintaining energy homeostasis in pathogen cells | Leptospira interrogans |