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Literature summary for 3.6.4.10 extracted from

  • Johnston, D.M.; Miot, M.; Hoskins, J.R.; Wickner, S.; Doyle, S.M.
    Substrate discrimination by ClpB and Hsp104 (2017), Front. Mol. Biosci., 4, 36 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.4.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Thermus thermophilus
expressed in Escherichia coli strain Rosetta(DE3) Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Saccharomyces cerevisiae
-
 ADP + phosphate
-
 ?
ATP + H2O Thermus thermophilus
-
 ADP + phosphate
-
 ?
additional information Thermus thermophilus the enzyme collaborates with the DnaK chaperone system to dissolve protein aggregates both in vivo and in vitro ?
-
-
additional information Saccharomyces cerevisiae the enzyme collaborates with the Hsp70 chaperone system to dissolve protein aggregates both in vivo and in vitro ?
-
-

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Thermus thermophilus Q9RA63
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography and Sephacryl S-200 gel filtration Saccharomyces cerevisiae
Q-Sepharose column chromatography and Sephacryl S-200 gel filtration Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Saccharomyces cerevisiae ADP + phosphate
-
 ?
ATP + H2O
-
 Thermus thermophilus ADP + phosphate
-
 ?
additional information the enzyme collaborates with the DnaK chaperone system to dissolve protein aggregates both in vivo and in vitro Thermus thermophilus ?
-
-
additional information the enzyme collaborates with the Hsp70 chaperone system to dissolve protein aggregates both in vivo and in vitro Saccharomyces cerevisiae ?
-
-
additional information the enzyme unfolds GFP-X30-H6,GFP-X7-H6, and GFP-15 with Km values of 0.0018 mM, 0.0052 mM, and 0.0016 mM, respectively Saccharomyces cerevisiae ?
-
-
additional information the enzyme unfolds GFP-X30-H6,GFP-X7-H6, and GFP-15 with Km values of 0.0041 mM, 0.003 mM, and 0.0012 mM, respectively Thermus thermophilus ?
-
-

Subunits

Subunits Comment Organism
hexamer
-
 Saccharomyces cerevisiae
hexamer
-
 Thermus thermophilus

Synonyms

Synonyms Comment Organism
ClpB
-
 Thermus thermophilus
Hsp104
-
 Saccharomyces cerevisiae

General Information

General Information Comment Organism
physiological function the enzyme is required for thermotolerance and function in disaggregation and reactivation of aggregated proteins that form during severe stress conditions Saccharomyces cerevisiae
physiological function the enzyme is required for thermotolerance and function in disaggregation and reactivation of aggregated proteins that form during severe stress conditions Thermus thermophilus