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Literature summary for 3.6.4.10 extracted from

  • Moro, F.; Fernandez-Saiz, V.; Slutsky, O.; Azem, A.; Muga, A.
    Conformational properties of bacterial DnaK and yeast mitochondrial Hsp70. Role of the divergent C-terminal alpha-helical subdomain (2005), FEBS J., 272, 3184-3196.
    View publication on PubMed
Search for more literature for 3.6.4.10

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli
-
 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information construction of DnaK /mtHsp70 chimeras by replacing different regions of the DnaK (from Escherichia coli) peptide-binding domain with those of mtHsp70 (from Saccharomyces cerevisiae) results in chimeric proteins that: (a) are not able to support growth of an Escherichia coli DnaK deletion strain at stress temperatures (e.g. 42°C), (b) show increased accessibility and decreased thermal stability of the peptide-binding pocket, and (c) have reduced activation by bacterial, but not mitochondrial cochaperones, as compared with DnaK Escherichia coli
additional information construction of DnaK /mtHsp70 chimeras by replacing different regions of the DnaK (from Escherichia coli) peptide-binding domain with those of mtHsp70 (from Saccharomyces cerevisiae) results in chimeric proteins that: (a) are not able to support growth of an Escherichia coli DnaK deletion strain at stress temperatures (e.g. 42°C), (b) show increased accessibility and decreased thermal stability of the peptide-binding pocket, and (c) have reduced activation by bacterial, but not mitochondrial cochaperones, as compared with DnaK Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Escherichia coli ADP + phosphate
-
 ?
ATP + H2O
-
 Saccharomyces cerevisiae ADP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
DnaK
-
 Escherichia coli
Hsp70
-
 Saccharomyces cerevisiae