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Literature summary for 3.6.2.1 extracted from
- Purification and properties of bovine liver lysosomal adenosine 5'-phosphosulphate sulphohydrolase. A nonspecific enzyme with pyrophosphatase and phosphodiesterase activities (1978), Biochim. Biophys. Acta, 527, 70-85.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
adenosine 5'-phosphosulfate | - |
Bos taurus |  |
| 0.14 | - |
ATP | - |
Bos taurus | |
| 0.14 | - |
phosphate | - |
Bos taurus | |
| 0.4 | - |
bis(4-nitrophenyl)phosphate | - |
Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| lysosome | - |
Bos taurus | 5764 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
SDS-PAGE | Bos taurus |
| 56000 | - |
gel filtration | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + H2O | Bos taurus | - |
? | - |
? | |
| ATP + H2O | Bos taurus | - |
? | - |
? | |
| diphosphate + H2O | Bos taurus | - |
2 phosphate | - |
? | |
| FAD + H2O | Bos taurus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 42.2 | - |
- |
Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenosine 5'-phosphosulphate + H2O | - |
Bos taurus | adenosine 3',5'-diphosphate + SO42- | - |
? | |
| adenosine 5'-phosphosulfate + H2O | - |
Bos taurus | AMP + SO42- | - |
? | |
| ADP + H2O | - |
Bos taurus | ? | - |
? | |
| ATP + H2O | - |
Bos taurus | ? | - |
? | |
| bis(4-nitrophenyl)phosphate + H2O | - |
Bos taurus | ? | - |
? | |
| diphosphate + H2O | - |
Bos taurus | 2 phosphate | - |
? | |
| FAD + H2O | - |
Bos taurus | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
stable for several months | Bos taurus |
- |
- |
stable for 30 h | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | ATP or phosphate as substrate | Bos taurus |
| 6 | - |
adenosine 5'-phosphosulfate as substrate | Bos taurus |
| 7 | - |
bis(4-nitrophenyl)phosphate as substrate | Bos taurus |
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