Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.1.B11 extracted from

  • Garza, J.A.; Ilangovan, U.; Hinck, A.P.; Barnes, L.D.
    Kinetic, dynamic, ligand binding properties, and structural models of a dual-substrate specific nudix hydrolase from Schizosaccharomyces pombe (2009), Biochemistry, 48, 6224-6239.
    View publication on PubMed
Search for more literature for 3.6.1.B11

Cloned(Commentary)

Cloned (Comment) Organism
cloning of His10-tagged aps1, aps1DELTA2-19, and aps1DELTA2-31, expression in Escherichia coli Schizosaccharomyces pombe

Protein Variants

Protein Variants Comment Organism
E93Qaps1DELTA2-19 mutant has 0.0003 times the hydrolase activity toward P1,P6-bis(5'-adenosyl)hexaphosphate as compared to wild-type enzyme Schizosaccharomyces pombe

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00219
-
 P1,P6-bis(5'-adenosyl)hexaphosphate pH 8.0, 37°C, His10-tagged truncated mutant enzyme DELTA2-31 Schizosaccharomyces pombe
0.00222
-
 P1,P6-bis(5'-adenosyl)hexaphosphate pH 8.0, 37°C, His10-tagged truncated mutant enzyme DELTA2-19 Schizosaccharomyces pombe
0.00284
-
 P1,P6-bis(5'-adenosyl)hexaphosphate pH 8.0, 37°C, wild-type enzyme Schizosaccharomyces pombe
0.00329
-
 P1,P6-bis(5'-adenosyl)hexaphosphate pH 8.0, 37°C, His10-tagged full length recombinant enzyme Schizosaccharomyces pombe

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type Aps1, His10-tagged Aps1, and truncated forms of Aps1 Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O degrades P1,P6-bis(5'-adenosyl)hexaphosphate and P1,P5-bis(5'-adenosyl)pentaphosphate, in preference to other diadenosine polyphosphates. The initial 31 residues of Aps1 are not involved in substrate binding or catalysis with respect to P1,P6-bis(5'-adenosyl)hexaphosphate Schizosaccharomyces pombe adenosine 5'-tetraphosphate + ADP
-
 ?

Synonyms

Synonyms Comment Organism
APS1
-
 Schizosaccharomyces pombe

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Schizosaccharomyces pombe

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.46
-
 P1,P6-bis(5'-adenosyl)hexaphosphate pH 8.0, 37°C, His10-tagged truncated mutant enzyme DELTA2-31 Schizosaccharomyces pombe
0.67
-
 P1,P6-bis(5'-adenosyl)hexaphosphate pH 8.0, 37°C, wild-type enzyme Schizosaccharomyces pombe
0.69
-
 P1,P6-bis(5'-adenosyl)hexaphosphate pH 8.0, 37°C, His10-tagged full length recombinant enzyme Schizosaccharomyces pombe
0.89
-
 P1,P6-bis(5'-adenosyl)hexaphosphate pH 8.0, 37°C, His10-tagged truncated mutant enzyme DELTA2-19 Schizosaccharomyces pombe

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Schizosaccharomyces pombe

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.23
-
 P1,P6-bis(5'-adenosyl)hexaphosphate pH 8.0, 37°C, His10-tagged truncated mutant enzyme DELTA2-31 Schizosaccharomyces pombe
4.67
-
 P1,P6-bis(5'-adenosyl)hexaphosphate pH 8.0, 37°C, His10-tagged truncated mutant enzyme DELTA2-19 Schizosaccharomyces pombe
21.2
-
 P1,P6-bis(5'-adenosyl)hexaphosphate pH 8.0, 37°C, His10-tagged full length recombinant enzyme Schizosaccharomyces pombe
23.7
-
 P1,P6-bis(5'-adenosyl)hexaphosphate pH 8.0, 37°C, wild-type enzyme Schizosaccharomyces pombe