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Literature summary for 3.6.1.9 extracted from

  • Jin, J.; Wu, R.; Zhu, J.; Yang, S.; Lei, Z.; Wang, N.; Singh, V.K.; Zheng, J.; Jia, Z.
    Insights into the cellular function of YhdE, a nucleotide pyrophosphatase from Escherichia coli (2015), PLoS ONE, 10, e0117823.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant E33A, hanging drop vapor diffusion method, using 0.1 Mmagnesium sulfate, 0.1 M MES buffer (pH 6.5), and 10-15% (w/v) PEG 8000 Escherichia coli

Protein Variants

Protein Variants Comment Organism
E33A inactive Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 dTTP at pH 7.0 and 25°C Escherichia coli
0.5
-
 UTP at pH 7.0 and 25°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 2 * 45000, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P25536
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-charged column chromatography and gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTTP + H2O the enzyme possesses dTTP-specific activity at a substrate concentration of 0.1 mM Escherichia coli dTMP + diphosphate
-
 ?
dUTP + H2O weakest substrate Escherichia coli dUMP + diphosphate
-
 ?
additional information no activity with ATP, CTP, GTP, TDP, TMP, and dCTP Escherichia coli ?
-
 ?
TTP + H2O the enzyme exhibits significant activity for TTP only when substrate concentration reaches 0.5 mM Escherichia coli TMP + diphosphate
-
 ?
UTP + H2O the enzyme exhibits significant activity for UTP only when substrate concentration reaches 0.5 mM Escherichia coli UMP + diphosphate
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 45000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
Maf-like protein YhdE
-
 Escherichia coli
nucleotide pyrophosphatase
-
 Escherichia coli
PPase
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
43
-
 dTTP at pH 7.0 and 25°C Escherichia coli
95
-
 UTP at pH 7.0 and 25°C Escherichia coli

General Information

General Information Comment Organism
malfunction enzyme overexpression results in filamentous cells. The enzyme plays a role in in cell growth inhibition under stress conditions, cell-division arrest and cell-shape maintenance Escherichia coli
physiological function emzyme-knockout cells transform the normal rod shape of wild type cells to a more spherical form, and the cell wall appears to become more flexible Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
191
-
 UTP at pH 7.0 and 25°C Escherichia coli
503
-
 dTTP at pH 7.0 and 25°C Escherichia coli