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Literature summary for 3.6.1.9 extracted from
- Biochemical characterization of an ectonucleotide pyrophosphatase/phosphodiesterase (E-NPP, E.C. 3.1.4.1) from rat cardiac soluble and microsomal fractions (2012), J. Enzyme Inhib. Med. Chem., 27, 29-36.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Rattus norvegicus |  |
| additional information | no inhibition by gadolinium chloride, levamisole, heparin, and lysophosphatidic acid in the soluble and microsomal fractions | Rattus norvegicus | |
| suramin | a P2 receptor antagonist and an inhibitor of E-NTPDase and NPP1-2 activities | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0919 | - |
4-nitrophenyl-5'-TMP | microsomal fraction, pH 8.9, 37°C | Rattus norvegicus | |
| 0.1185 | - |
4-nitrophenyl-5'-TMP | soluble fraction, pH 8.9, 37°C | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | higher activity than in the soluble fraction | Rattus norvegicus | - |
- |
| soluble | lower activity than in the microsomal fraction | Rattus norvegicus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Rattus norvegicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
male | - |
| Rattus norvegicus Wistar | - |
male | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl-5'-TMP + H2O | - |
Rattus norvegicus | 4-nitrophenol + 5'-TMP | - |
? | |
| 4-nitrophenyl-5'-TMP + H2O | - |
Rattus norvegicus Wistar | 4-nitrophenol + 5'-TMP | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| E-NPP | - |
Rattus norvegicus |
| ectonucleotide pyrophosphatase/phosphodiesterase | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.9 | - |
soluble fraction | Rattus norvegicus |
| 9.3 | - |
microsomal fraction | Rattus norvegicus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme might be involved in nucleotide signalling in the cardiac tissue | Rattus norvegicus |
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