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Literature summary for 3.6.1.9 extracted from

  • Joye, I.; Belien, T.; Brijs, K.; Proost, P.; Soetaert, W.; Delcour, J.
    Characterisation of the first wheat (Triticum aestivum L.) nucleotide pyrophosphatase/phosphodiesterase resembling mammalian counterparts (2010), J. Cereal Sci., 51, 326-336.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, cloning in Escherichia coli TOP10 cells, and overexpression as His6-tagged protein in Escherichia coli strain BL21 (DE3)pLysS cells using pEXP5-CT/TOPO vector. Expression in Pichia pastoris strain X-33 Triticum aestivum

Inhibitors

Inhibitors Comment Organism Structure
AMP
-
Triticum aestivum
EDTA activity is restorable by Zn2+ Triticum aestivum
additional information poor inhibition by imidazole at 2 mM Triticum aestivum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Triticum aestivum

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane contains a putative transmembrane domain Triticum aestivum 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ activates Triticum aestivum

Organism

Organism UniProt Comment Textmining
Triticum aestivum D9YT79 cf. EC 3.1.4.1
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from wheat germs, recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography Triticum aestivum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
188.2
-
purified recombinant enzyme, pH 8.7, 35°C Triticum aestivum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-5'-TMP + H2O
-
Triticum aestivum 4-nitrophenol + 5'-TMP
-
?
ADP-ribose + H2O best substrate Triticum aestivum AMP + phosphoribose
-
?
ATP + H2O low activity Triticum aestivum ADP + phosphate
-
?
CTP + H2O low activity Triticum aestivum CDP + phosphate
-
?
GTP + H2O low activity Triticum aestivum GDP + phosphate
-
?
NAD+ + H2O
-
Triticum aestivum NMN + AMP
-
?
NADH + H2O
-
Triticum aestivum NMNH + AMP
-
?
NADP+ + H2O
-
Triticum aestivum NMN + adenosine 2',5'-phosphate
-
?
NADPH + H2O high activity Triticum aestivum NMNH + adenosine 2',5'-phosphate
-
?
TTP + H2O low activity Triticum aestivum TDP + phosphate
-
?

Subunits

Subunits Comment Organism
oligomer
-
Triticum aestivum

Synonyms

Synonyms Comment Organism
NPP
-
Triticum aestivum
nucleotide pyrophosphatase/phosphodiesterase
-
Triticum aestivum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Triticum aestivum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
purified enzyme, pH 8.7, 30 min, stable, 75% activity remaining after 90 min Triticum aestivum
80
-
purified enzyme, pH 8.7, half-life is 5 min Triticum aestivum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
12
-
-
Triticum aestivum

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 12 purified enzyme, 35°C, 30 min, over 75% activity remaining Triticum aestivum