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Literature summary for 3.6.1.9 extracted from
- The purification and properties of detergent-solubilized rat liver nucleotide pyrophosphatase (1972), J. Biol. Chem., 247, 5071-5078.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5'-AMP | 29% inhibition at 12 mM | Rattus sp. |  |
| dAMP | 16% inhibition at 17 mM | Rattus sp. | |
| diphosphate | 26% inhibition at 10 mM | Rattus sp. | |
| EDTA | 50% inhibition at 1 mM | Rattus sp. | |
| NaF | 22% inhibition at 45 mM | Rattus sp. | |
| UMP | 11% inhibition at 56 mM | Rattus sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.6 | - |
NADH | - |
Rattus sp. | |
| 14.9 | - |
UDP-glucose | - |
Rattus sp. | |
| 18.9 | - |
ADP-ribose | - |
Rattus sp. | |
| 31.2 | - |
NAD+ | - |
Rattus sp. | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Rattus sp. | 16020 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-ribose + H2O | Rattus sp. | - |
AMP + phosphoribose | - |
? | |
| ATP + H2O | Rattus sp. | - |
AMP + diphosphate | - |
? | |
| NAD+ + H2O | Rattus sp. | - |
NMN + AMP | - |
? | |
| NADH + H2O | Rattus sp. | - |
NMNH + AMP | - |
? | |
| NADPH + H2O | Rattus sp. | - |
NMNH + adenosine 2',5'-phosphate | - |
? | |
| UDP-glucose + H2O | Rattus sp. | - |
UMP + D-glucose-1-phosphate | - |
? | |
| UDP-glucuronic acid + H2O | Rattus sp. | - |
UMP + glucuronic acid phosphate | - |
? | |
| UDP-N-acetyl-alpha-D-glucosamine + H2O | Rattus sp. | - |
UMP + N-acetyl-alpha-D-glucosaminyl-1-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Rattus sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.1 | - |
- |
Rattus sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-ribose + H2O | - |
Rattus sp. | AMP + phosphoribose | - |
? | |
| ATP + H2O | - |
Rattus sp. | AMP + diphosphate | - |
? | |
| bis(p-nitrophenyl)phosphate + H2O | 0.31% hydrolysis rate compared to NADH as substrate | Rattus sp. | p-nitrophenol + p-nitrophenylphosphate | - |
? | |
| NAD+ + H2O | - |
Rattus sp. | NMN + AMP | - |
? | |
| NADH + H2O | - |
Rattus sp. | NMNH + AMP | - |
? | |
| NADPH + H2O | - |
Rattus sp. | NMNH + adenosine 2',5'-phosphate | - |
? | |
| p-nitrophenylphosphate + H2O | 0.04% hydrolysis rate compared to NADH as substrate | Rattus sp. | p-nitrophenol + phosphate | - |
? | |
| UDP-glucose + H2O | - |
Rattus sp. | UMP + D-glucose-1-phosphate | - |
? | |
| UDP-glucuronic acid + H2O | - |
Rattus sp. | UMP + glucuronic acid phosphate | - |
? | |
| UDP-N-acetyl-alpha-D-glucosamine + H2O | - |
Rattus sp. | UMP + N-acetyl-alpha-D-glucosaminyl-1-phosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Rattus sp. |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | completely stable between, less stable at lower pH | Rattus sp. |
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