Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 19% inhibition at 4 mM | Saccharomyces cerevisiae | |
dithiothreitol | 92% inhibition at 4 mM, reversal to 92% of initial activity by addition of Zn2+ | Saccharomyces cerevisiae | |
EDTA | complete inactivation at 2 mM, no reversal by addition of metals | Saccharomyces cerevisiae | |
L-ascorbic acid | 8% inhibition at 16 mM | Saccharomyces cerevisiae | |
L-cysteine | complete inhibition at 4 mM, reversal to 95% of initial activity by addition of Zn2+ | Saccharomyces cerevisiae | |
o-phenanthroline | complete inactivation at 2 mM, no reversal by addition of metal ions, m-phenanthroline no effect | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | tightly bound, contains 1 zinc atom per mol of enzyme | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-mannose + H2O | Saccharomyces cerevisiae | - |
GMP + D-mannose-1-phosphate | - |
? | |
UDP-glucose + H2O | Saccharomyces cerevisiae | - |
UMP + D-glucose-1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
baker's yeast | - |
Purification (Comment) | Organism |
---|---|
chromatography techniques | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-mannose + H2O | - |
Saccharomyces cerevisiae | GMP + D-mannose-1-phosphate | - |
? | |
p-nitrophenyl-dTMP + H2O | - |
Saccharomyces cerevisiae | p-nitrophenol + dTMP | - |
? | |
UDP-glucose + H2O | - |
Saccharomyces cerevisiae | UMP + D-glucose-1-phosphate | - |
? |