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Literature summary for 3.6.1.9 extracted from
- Nucleotide pyrophosphatase from potato tubers. Purification and properties (1984), Eur. J. Biochem., 143, 419-426.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2',3'-cAMP | 7% inhibition at 2 mM | Solanum tuberosum |  |
| 3',5'-cAMP | 4% inhibition at 2 mM | Solanum tuberosum | |
| 5'-AMP | 56% inhibition at 2 mM | Solanum tuberosum | |
| adenosine 2'-monophosphate | 8% inhibition at 2 mM | Solanum tuberosum | |
| adenosine 3'-monophosphate | 8% inhibition at 2 mM | Solanum tuberosum | |
| alpha,beta-methylene adenosine 5'-triphosphate | 25% inhibition at 2 mM | Solanum tuberosum | |
| ATP | 44% inhibition at 2 mM | Solanum tuberosum | |
| diphosphate | 8% inhibition at 2 mM | Solanum tuberosum | |
| NAD+ | 84% inhibition at 2 mM | Solanum tuberosum | |
| phosphate | 18% inhibition at 2 mM | Solanum tuberosum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
NAD+ | - |
Solanum tuberosum | |
| 0.05 | - |
5',5'-diadenosine diphosphate | - |
Solanum tuberosum | |
| 0.05 | - |
5',5'-diadenosine triphosphate | - |
Solanum tuberosum | |
| 0.1 | - |
7-methyl 5'-guanosine 2'-O-methylguanosine triphosphate | - |
Solanum tuberosum | |
| 0.2 | - |
5',5'-diadenosine tetraphosphate | - |
Solanum tuberosum | |
| 0.5 | - |
ATP | - |
Solanum tuberosum | |
| 0.5 | - |
dATP | - |
Solanum tuberosum | |
| 1 | - |
thymidine 5'-monophosphate p-nitrophenyl ester | - |
Solanum tuberosum | |
| 1.3 | - |
thymidine 5'-diphospho 5'-thymidine | - |
Solanum tuberosum | |
| 2.3 | - |
2',3'-cAMP | - |
Solanum tuberosum | |
| 2.7 | - |
thymidine 3'-diphospho 3'-thymidine | - |
Solanum tuberosum | |
| 6.1 | - |
thymidine 3'-monophosphate p-nitrophenyl ester | - |
Solanum tuberosum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 74000 | - |
4 * 74000, SDS-PAGE | Solanum tuberosum |
| 346000 | - |
sucrose density gradient centrifugation | Solanum tuberosum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2',3'-cAMP + H2O | Solanum tuberosum | low activity | adenosine monophosphate | - |
? | |
| 3',5'-cAMP + H2O | Solanum tuberosum | traces of activity | adenosine monophosphate | - |
? | |
| 5'-AMP + H2O | Solanum tuberosum | traces of activity | adenosine + phosphate | - |
? | |
| ADP + H2O | Solanum tuberosum | low activity | AMP + phosphate | - |
? | |
| ATP + H2O | Solanum tuberosum | - |
AMP + diphosphate | initial product: AMP | ? | |
| CDP + H2O | Solanum tuberosum | low activity | CMP + phosphate | - |
? | |
| CTP + H2O | Solanum tuberosum | low activity | ? | - |
? | |
| dATP + H2O | Solanum tuberosum | - |
? | - |
? | |
| mRNA + H2O | Solanum tuberosum | reovirus m7-G(5')p3(5')Gm-terminated intact mRNA | 7-methyl-GMP + ? | - |
? | |
| NAD+ + H2O | Solanum tuberosum | - |
NMN + AMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum tuberosum | - |
potato | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Solanum tuberosum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography techniques | Solanum tuberosum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| tuber | - |
Solanum tuberosum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2831 | - |
- |
Solanum tuberosum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2',3'-cAMP + H2O | low activity | Solanum tuberosum | adenosine monophosphate | - |
? | |
| 3',5'-cAMP + H2O | traces of activity | Solanum tuberosum | adenosine monophosphate | - |
? | |
| 5',5'-diadenosine tetraphosphate + H2O | - |
Solanum tuberosum | ATP + 5'-AMP | - |
? | |
| 5',5'-diadenosine triphosphate + H2O | - |
Solanum tuberosum | ADP + 5'-AMP | - |
? | |
| 5'-AMP + H2O | traces of activity | Solanum tuberosum | adenosine + phosphate | - |
? | |
| 7-methyl 5'-guanosine 2'-O-methylguanosine triphosphate + H2O | - |
Solanum tuberosum | 7-methyl 5'-guanosine 2'-O-methylguanosine diphosphate + phosphate | - |
? | |
| 7-methyl 5'-guanosine 2'-O-methylgunaosine triphosphate + H2O | - |
Solanum tuberosum | 7-methyl-GMP + 7-methyl-GDP + GMP + GDP | - |
? | |
| 8-bromo-NAD+ + H2O | same hydrolysis rate as for NAD+ | Solanum tuberosum | 8-bromo-AMP + NMN | - |
? | |
| adenosine (alpha,beta-methylene) 5'-triphosphate + H2O | - |
Solanum tuberosum | adenosine (alpha,beta-methylene) 5'-diphosphate + phosphate | - |
? | |
| ADP + H2O | low activity | Solanum tuberosum | AMP + phosphate | - |
? | |
| ATP + H2O | - |
Solanum tuberosum | AMP + diphosphate | initial product: AMP | ? | |
| beta-naphthylphenylphosphonate + H2O | very low activity | Solanum tuberosum | ? | - |
? | |
| bis(p-nitrophenyl)phosphate + H2O | - |
Solanum tuberosum | p-nitrophenol + p-nitrophenylphosphate | - |
? | |
| CDP + H2O | low activity | Solanum tuberosum | CMP + phosphate | - |
? | |
| CTP + H2O | low activity | Solanum tuberosum | ? | - |
? | |
| dATP + H2O | - |
Solanum tuberosum | ? | - |
? | |
| diadenosine 5'-diphosphate + H2O | - |
Solanum tuberosum | adenosine 5'-monophosphate + ? | - |
? | |
| diadenosine polyphosphate + H2O | from di- to pentaphosphates, hydrolysis rate decreases with chain length | Solanum tuberosum | AMP + ? | - |
? | |
| guanosine (alpha,beta-methylene) 5'-triphosphate + H2O | - |
Solanum tuberosum | guanosine (alpha,beta-methylene) 5'-diphosphate + phosphate | - |
? | |
| mRNA + H2O | reovirus m7-G(5')p3(5')Gm-terminated intact mRNA | Solanum tuberosum | 7-methyl-GMP + ? | - |
? | |
| NAD+ + H2O | - |
Solanum tuberosum | NMN + AMP | - |
? | |
| p-nitrophenyl phenylphosphonate + H2O | low activity | Solanum tuberosum | p-nitrophenol + phenylphosphonate | - |
? | |
| p-nitrophenylphosphate + H2O | - |
Solanum tuberosum | p-nitrophenol + phosphate | - |
? | |
| thymidine 3'-diphospho 3'-thymidine + H2O | very low activity | Solanum tuberosum | thymidine 3'-phosphate + thymidine 3'-phosphate | - |
? | |
| thymidine 3'-monophosphate p-nitrophenyl ester + H2O | low activity | Solanum tuberosum | thymidine 3'-monophosphate + p-nitrophenol | - |
? | |
| thymidine 5'-6-bromo-2-hydroxy-3-naphthoic acid 2-methoxyanilide-naphthylphosphate + H2O | low activity | Solanum tuberosum | ? | - |
? | |
| thymidine 5'-alpha-naphthylphosphate + H2O | very low activity | Solanum tuberosum | thymidine + alpha-naphthylphosphate | - |
? | |
| thymidine 5'-diphospho 5'-thymidine + H2O | low activity | Solanum tuberosum | thymidine 5'-phosphate + thymidine 5'-phosphate | - |
? | |
| thymidine 5'-monophosphate p-nitrophenyl ester + H2O | - |
Solanum tuberosum | thymidine 5'-monophosphate + p-nitrophenol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 74000, SDS-PAGE | Solanum tuberosum |
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