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Literature summary for 3.6.1.9 extracted from

  • Grobben, B.; Anciaux, K.; Roymans, D.; Stefan, C.; Bollen, M.; Esmans, E.L.; Slegers, H.
    An ecto-nucleotide pyrophosphatase is one of the main enzymes involved in the extracellular metabolism of ATP in rat C6 glioma (1999), J. Neurochem., 72, 826-834.
    View publication on PubMed
Search for more literature for 3.6.1.9

Activating Compound

Activating Compound Comment Organism Structure
pyridoxal phosphate-6-azophenyl-2',4'disulfonic acid slight stimulation below 0.02 mM Rattus sp.

Inhibitors

Inhibitors Comment Organism Structure
adenosine 3',5'-bisphosphate
-
 Rattus sp.
EDTA abolishes acivity Rattus sp.
nucleoside monophosphates
-
 Rattus sp.
pyridoxal phosphate-6-azophenyl-2',4'disulfonic acid complete inhibition above 0.02 mM Rattus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 ATP
-
 Rattus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
 Rattus sp. 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ slight increase of activity Rattus sp.
Mg2+ slight increase of activity Rattus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Rattus sp. accounts for 50-75% of extracellular hydrolysed ATP AMP + diphosphate
-
 ?
GTP + H2O Rattus sp. 1.5fold more hydrolysis than for ATP GMP + diphosphate
-
 ?
NAD+ + H2O Rattus sp.
-
 NMN + AMP
-
 ?
UTP + H2O Rattus sp. 2fold more hydrolysis than for ATP UMP + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus sp.

Reaction

Reaction Comment Organism Reaction ID
a nucleoside triphosphate + H2O = a nucleotide + diphosphate substrates include NAD+, NADP+, FAD, CoA and also ATP and ADP Rattus sp.
a

Source Tissue

Source Tissue Comment Organism Textmining
glioma cell homologue to plasma cell membrane glycoprotein PC-1 Rattus sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenosine 5'-phosphosulfate + H2O
-
 Rattus sp. 3'-phosphoadenosine + phosphosulfate
-
 ?
5'-adenylylimidodiphosphate + H2O
-
 Rattus sp. ?
-
 ?
adenosine 5'-O-(2-thiotriphosphate) + H2O
-
 Rattus sp. AMP + ?
-
 ?
ATP + H2O accounts for 50-75% of extracellular hydrolysed ATP Rattus sp. AMP + diphosphate
-
 ?
beta,gamma-methyleneadenosine 5'-triphosphate + H2O
-
 Rattus sp. adenosine 5'-monophosphate + phosphomethylposphoric acid
-
 ?
GTP + H2O 1.5fold more hydrolysis than for ATP Rattus sp. GMP + diphosphate
-
 ?
NAD+ + H2O
-
 Rattus sp. NMN + AMP
-
 ?
thymidine 5'-monophosphate p-nitrophenyl ester + H2O
-
 Rattus sp. thymidine 5'-monophosphate + p-nitrophenol
-
 ?
UTP + H2O 2fold higher hydrolysis rate than for ATP Rattus sp. UMP + diphosphate
-
 ?
UTP + H2O 2fold more hydrolysis than for ATP Rattus sp. UMP + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
ecto-nucleotide pyrophosphatase
-
 Rattus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
 Rattus sp.