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Literature summary for 3.6.1.9 extracted from

  • Byrd, J.C.; Fearney, F.J.; Kim, Y.S.
    Rat intestinal nucleotide-sugar pyrophosphatase. Localization, partial purification, and substrate specificity (1985), J. Biol. Chem., 260, 7474-7480.
    View publication on PubMed
Search for more literature for 3.6.1.9

Inhibitors

Inhibitors Comment Organism Structure
2,3-dimercapto-1-propanol complete inhibition at 5 mM Rattus sp.
Adenyl-5'-yl imidodiphosphate 61-78% inhibition at 1 mM Rattus sp.
alpha,beta-methylene adenosine 5'-triphosphate 61-78% inhibition at 0.3 mM Rattus sp.
CDP-choline 62% inhibition at 0.2 mM Rattus sp.
coenzyme A 90% inhibition at 0.2 mM Rattus sp.
EDTA inactivation after 30 min preincubation at 6 mM Rattus sp.
FAD+ 79% inhibition at 0.2 mM Rattus sp.
NAD+ 82% inhibition at 0.2 mM Rattus sp.
p-nitrophenyl 3'-thymidylate 15% inhibition at 0.2 mM Rattus sp.
p-nitrophenyl 5'-thymidylate 15% inhibition at 0.2 mM Rattus sp.
thiamine diphosphate 17% inhibition at 0.2 mM Rattus sp.
UDP-glucose competitive inhibition of phosphodiesterase activity Rattus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 GDP-mannose
-
 Rattus sp.
0.21
-
 p-nitrophenyl 5'-TMP
-
 Rattus sp.
1.23
-
 FAD
-
 Rattus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
brush border
-
 Rattus sp. 5903
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
FAD + H2O Rattus sp.
-
 FMN + AMP
-
 ?
GDP-mannose + H2O Rattus sp.
-
 GMP + D-mannose-1-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
intestine
-
 Rattus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.7
-
-
 Rattus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FAD + H2O
-
 Rattus sp. FMN + AMP
-
 ?
GDP-mannose + H2O
-
 Rattus sp. GMP + D-mannose-1-phosphate
-
 ?
p-nitrophenyl-dTMP + H2O
-
 Rattus sp. p-nitrophenol + dTMP
-
 ?
thymidine 5'-monophosphate p-nitrophenyl ester + H2O
-
 Rattus sp. thymidine 5'-monophosphate + p-nitrophenol
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 half-life: 6.4-10.4 min Rattus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.5
-
 Rattus sp.