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Literature summary for 3.6.1.9 extracted from

  • Uriarte, M.; Stalmans, W.; Hickman, S.; Bollen, M.
    Regulation of purified hepatic PC-1 (phosphodiesterase-I/nucleotide pyrophosphatase) by threonine auto(de)phosphorylation and by binding of acidic fibroblast growth factor (1995), Biochem. J., 306, 271-277.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.9

Inhibitors

Inhibitors Comment Organism Structure
acidic fibroblast growth factor potent inhibition at nearly stoichiometric concentrations, Ki: 1.3 nM Rattus sp.
dithiothreitol 50% inhibition at 2.3 mM Rattus sp.
EDTA complete inhibition at 1 mM Rattus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Rattus sp. 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
118000
-
 2 * 118000 + 2 * 128000, SDS-PAGE Rattus sp.
128000
-
 2 * 118000 + 2 * 128000, SDS-PAGE Rattus sp.
500000
-
 gel filtration Rattus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Rattus sp.
-
 AMP + diphosphate little accumulation of ADP ?
UDP-glucose + H2O Rattus sp.
-
 UMP + D-glucose-1-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein autophosphorylation by protein threonin kinase activity with intrinsic dephosphorylase activity Rattus sp.

Purification (Commentary)

Purification (Comment) Organism
to homogemeity, chromatography steps, trifunctional enzyme, displaying phosphodiesterase-1/diphosphatase- and protein threonin kinase activity, that also phosphorylates histone IIa Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
411
-
 phosphodiesterase activity Rattus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Rattus sp. AMP + diphosphate little accumulation of ADP ?
thymidine 5'-monophosphate p-nitrophenyl ester + H2O significant phosphodiesterase activity Rattus sp. thymidine 5'-monophosphate + p-nitrophenol
-
 ?
UDP-glucose + H2O
-
 Rattus sp. UMP + D-glucose-1-phosphate
-
 ?

Subunits

Subunits Comment Organism
tetramer 2 * 118000 + 2 * 128000, SDS-PAGE Rattus sp.

Synonyms

Synonyms Comment Organism
nucleotide pyrophosphatase/phosphodiesterase-I
-
 Rattus sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000013
-
 acidic fibroblast growth factor potent inhibition at nearly stoichiometric concentrations Rattus sp.
2.3
-
 dithiothreitol
-
 Rattus sp.