Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acidic fibroblast growth factor | potent inhibition at nearly stoichiometric concentrations, Ki: 1.3 nM | Rattus sp. | |
dithiothreitol | 50% inhibition at 2.3 mM | Rattus sp. | |
EDTA | complete inhibition at 1 mM | Rattus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Rattus sp. | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
118000 | - |
2 * 118000 + 2 * 128000, SDS-PAGE | Rattus sp. |
128000 | - |
2 * 118000 + 2 * 128000, SDS-PAGE | Rattus sp. |
500000 | - |
gel filtration | Rattus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Rattus sp. | - |
AMP + diphosphate | little accumulation of ADP | ? | |
UDP-glucose + H2O | Rattus sp. | - |
UMP + D-glucose-1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus sp. | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | autophosphorylation by protein threonin kinase activity with intrinsic dephosphorylase activity | Rattus sp. |
Purification (Comment) | Organism |
---|---|
to homogemeity, chromatography steps, trifunctional enzyme, displaying phosphodiesterase-1/diphosphatase- and protein threonin kinase activity, that also phosphorylates histone IIa | Rattus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
411 | - |
phosphodiesterase activity | Rattus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Rattus sp. | AMP + diphosphate | little accumulation of ADP | ? | |
thymidine 5'-monophosphate p-nitrophenyl ester + H2O | significant phosphodiesterase activity | Rattus sp. | thymidine 5'-monophosphate + p-nitrophenol | - |
? | |
UDP-glucose + H2O | - |
Rattus sp. | UMP + D-glucose-1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * 118000 + 2 * 128000, SDS-PAGE | Rattus sp. |
Synonyms | Comment | Organism |
---|---|---|
nucleotide pyrophosphatase/phosphodiesterase-I | - |
Rattus sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000013 | - |
acidic fibroblast growth factor | potent inhibition at nearly stoichiometric concentrations | Rattus sp. | |
2.3 | - |
dithiothreitol | - |
Rattus sp. |