BRENDA - Enzyme Database
show all sequences of 3.6.1.8

Characterization of a RecA/RAD51 homologue from the hyperthermophilic archaeon Pyrococcus sp. KOD1

Rashid, N.; Morikawa, M.; Nagahisa, K.; Kanaya, S.; Imanaka, T.; Nucleic Acids Res. 25, 719-726 (1997)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Thermococcus kodakarensis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Zn2+
preferable divalent cation for ATPase activity
Thermococcus kodakarensis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
2 * 28000, SDS-PAGE
Thermococcus kodakarensis
49000
-
gel filtration
Thermococcus kodakarensis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermococcus kodakarensis
P95547
-
-
Purification (Commentary)
Commentary
Organism
-
Thermococcus kodakarensis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + H2O
the enzyme shows DNase activity on both single- and double-stranded DNAs along with the ATPase activity. ATPase activity is independent of both single-stranded and double-stranded DNAs
726055
Thermococcus kodakarensis
AMP + diphosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 28000, SDS-PAGE
Thermococcus kodakarensis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
55
-
assay at
Thermococcus kodakarensis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Thermococcus kodakarensis
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Thermococcus kodakarensis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Zn2+
preferable divalent cation for ATPase activity
Thermococcus kodakarensis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
2 * 28000, SDS-PAGE
Thermococcus kodakarensis
49000
-
gel filtration
Thermococcus kodakarensis
Purification (Commentary) (protein specific)
Commentary
Organism
-
Thermococcus kodakarensis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + H2O
the enzyme shows DNase activity on both single- and double-stranded DNAs along with the ATPase activity. ATPase activity is independent of both single-stranded and double-stranded DNAs
726055
Thermococcus kodakarensis
AMP + diphosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 28000, SDS-PAGE
Thermococcus kodakarensis
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
55
-
assay at
Thermococcus kodakarensis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Thermococcus kodakarensis
Other publictions for EC 3.6.1.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
668024
Martins
Inhibition of the ecto-ATPdiph ...
Schistosoma mansoni
Biosci. Rep.
20
369-381
2000
-
-
-
-
-
-
10
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
10
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726055
Rashid
Characterization of a RecA/RAD ...
Thermococcus kodakarensis
Nucleic Acids Res.
25
719-726
1997
-
-
1
-
-
-
-
-
-
1
2
-
-
4
-
-
1
-
-
-
-
-
1
1
1
-
-
-
1
-
-
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-
-
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-
1
-
-
-
-
-
-
-
-
-
1
2
-
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-
-
1
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
210227
Masuda
A unique ectonucleotide pyroph ...
Sus scrofa
J. Clin. Invest.
95
699-704
1995
-
-
-
-
-
-
-
2
2
-
2
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
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-
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-
-
-
-
-
-
-
-
-
-
2
2
-
2
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
210226
Capito
Presence of ATP-pyrophosphohyd ...
Mus musculus
Diabetes
35
1096-1100
1986
6
-
-
-
-
-
1
3
-
7
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
1
-
3
-
7
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
210225
Torp-Pedersen
Studies on a Ca2+-dependent nu ...
Rattus norvegicus
Biochim. Biophys. Acta
571
94-104
1979
-
-
-
-
-
-
2
2
1
4
1
2
-
2
-
-
-
-
-
3
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
2
1
4
1
2
-
-
-
-
-
3
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
210224
Flodgaard
A calcium ion-dependent adenos ...
Rattus norvegicus
Biochem. J.
171
817-820
1978
-
-
-
-
-
-
-
-
1
4
-
1
-
2
-
-
1
-
-
2
1
-
3
-
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-
-
-
-
-
-
-
-
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-
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-
-
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-
-
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-
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-
1
4
-
1
-
-
-
1
-
2
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
210223
Kawamura
Change in ATP-pyrophosphohydro ...
Physarum polycephalum
Biochim. Biophys. Acta
421
195-202
1976
-
-
-
-
-
-
2
-
-
-
-
1
-
2
-
-
1
-
-
1
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1
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2
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1
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-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
210222
Kawamura
A calcium ion-dependent ATP py ...
Physarum polycephalum
Biochim. Biophys. Acta
397
207-219
1975
-
-
-
-
-
-
1
1
-
8
2
3
-
3
-
-
-
-
-
1
-
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
8
2
3
-
-
-
-
-
1
-
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
210221
Heppel
Mechanism of enzymatic hydroly ...
Bos taurus, Crotalus adamanteus, Oryctolagus cuniculus, Rattus norvegicus
J. Biol. Chem.
202
217-226
1953
-
-
-
-
-
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-
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-
4
-
5
-
4
-
-
1
-
-
2
-
-
5
-
-
-
1
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
5
-
-
-
1
-
2
-
-
5
-
-
-
1
-
1
2
-
-
-
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-
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-
-