BRENDA - Enzyme Database
show all sequences of 3.6.1.8

Inhibition of the ecto-ATPdiphosphohydrolase of Schistosoma mansoni by thapsigargin

Martins, S.M.; Torres, C.R.; Ferreira, S.T.; Biosci. Rep. 20, 369-381 (2000)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
15-desoxybludein
0.065 mg/ml, 11% inhibition
Schistosoma mansoni
eremantolide C
0.065 mg/ml, 17% inhibition
Schistosoma mansoni
glaucolide A
0.065 mg/ml, 11% inhibition
Schistosoma mansoni
glaucolide B
0.065 mg/ml, 16% inhibition
Schistosoma mansoni
goyazensolide
0.065 mg/ml, 10% inhibition
Schistosoma mansoni
isogoyazensolide
0.065 mg/ml, 11% inhibition
Schistosoma mansoni
licnofolide
0.065 mg/ml, 22% inhibition
Schistosoma mansoni
additional information
no inhibition by 0.065 mg/ml 15-desoxygoyazensolide
Schistosoma mansoni
thapsigargicin
0.065 mg/ml, 57% inhibition
Schistosoma mansoni
thapsigargin
inhibits ATP hydrolysis, 70% inhibition at 0.065 mg/ml, mixed type inhibition, little or not affected by changes in free Ca2+ or Mg2+ concentrations, hydrolysis of ADP is not inhibited
Schistosoma mansoni
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0173
-
ATP
37°C, pH 7.4
Schistosoma mansoni
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Schistosoma mansoni
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + H2O
-
668024
Schistosoma mansoni
AMP + diphosphate
-
-
-
?
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.02
-
thapsigargin
37°C, pH 7.4, inhibition of ATP hydrolysis
Schistosoma mansoni
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
15-desoxybludein
0.065 mg/ml, 11% inhibition
Schistosoma mansoni
eremantolide C
0.065 mg/ml, 17% inhibition
Schistosoma mansoni
glaucolide A
0.065 mg/ml, 11% inhibition
Schistosoma mansoni
glaucolide B
0.065 mg/ml, 16% inhibition
Schistosoma mansoni
goyazensolide
0.065 mg/ml, 10% inhibition
Schistosoma mansoni
isogoyazensolide
0.065 mg/ml, 11% inhibition
Schistosoma mansoni
licnofolide
0.065 mg/ml, 22% inhibition
Schistosoma mansoni
additional information
no inhibition by 0.065 mg/ml 15-desoxygoyazensolide
Schistosoma mansoni
thapsigargicin
0.065 mg/ml, 57% inhibition
Schistosoma mansoni
thapsigargin
inhibits ATP hydrolysis, 70% inhibition at 0.065 mg/ml, mixed type inhibition, little or not affected by changes in free Ca2+ or Mg2+ concentrations, hydrolysis of ADP is not inhibited
Schistosoma mansoni
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.02
-
thapsigargin
37°C, pH 7.4, inhibition of ATP hydrolysis
Schistosoma mansoni
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0173
-
ATP
37°C, pH 7.4
Schistosoma mansoni
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + H2O
-
668024
Schistosoma mansoni
AMP + diphosphate
-
-
-
?
Other publictions for EC 3.6.1.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
668024
Martins
Inhibition of the ecto-ATPdiph ...
Schistosoma mansoni
Biosci. Rep.
20
369-381
2000
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1
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726055
Rashid
Characterization of a RecA/RAD ...
Thermococcus kodakarensis
Nucleic Acids Res.
25
719-726
1997
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-
1
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1
2
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4
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1
1
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1
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210227
Masuda
A unique ectonucleotide pyroph ...
Sus scrofa
J. Clin. Invest.
95
699-704
1995
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2
2
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2
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1
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2
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2
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1
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1
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1
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210226
Capito
Presence of ATP-pyrophosphohyd ...
Mus musculus
Diabetes
35
1096-1100
1986
6
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1
3
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7
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1
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1
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1
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6
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3
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7
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1
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1
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1
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210225
Torp-Pedersen
Studies on a Ca2+-dependent nu ...
Rattus norvegicus
Biochim. Biophys. Acta
571
94-104
1979
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-
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2
2
1
4
1
2
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2
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3
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4
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1
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210224
Flodgaard
A calcium ion-dependent adenos ...
Rattus norvegicus
Biochem. J.
171
817-820
1978
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4
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2
1
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3
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210223
Kawamura
Change in ATP-pyrophosphohydro ...
Physarum polycephalum
Biochim. Biophys. Acta
421
195-202
1976
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2
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1
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1
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210222
Kawamura
A calcium ion-dependent ATP py ...
Physarum polycephalum
Biochim. Biophys. Acta
397
207-219
1975
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1
1
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8
2
3
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3
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2
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4
1
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210221
Heppel
Mechanism of enzymatic hydroly ...
Bos taurus, Crotalus adamanteus, Oryctolagus cuniculus, Rattus norvegicus
J. Biol. Chem.
202
217-226
1953
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4
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5
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4
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5
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