BRENDA - Enzyme Database
show all sequences of 3.6.1.8

A unique ectonucleotide pyrophosphohyrolase associated with porcine chondrocyte-derived vesicels

Masuda, I.; Hamada, J.; Haas, A.L.; Ryan, L.M.; McCarthy, D.J.; J. Clin. Invest. 95, 699-704 (1995)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.83
-
ATP
vesicle associated enzyme at pH 7.3
Sus scrofa
1.28
-
ATP
vesicle associated enzyme at pH 9.9
Sus scrofa
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
human erythrocyte membrane
Sus scrofa
16020
-
soluble
two different localisations found
Sus scrofa
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
61000
-
SDS-PAGE, soluble enzyme
Sus scrofa
127000
-
SDS-PAGE, sedimentable enzyme
Sus scrofa
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sus scrofa
-
-
-
Purification (Commentary)
Commentary
Organism
-
Sus scrofa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + H2O
-
210227
Sus scrofa
AMP + diphosphate
-
210227
Sus scrofa
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.9
-
particular enzymes
Sus scrofa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.83
-
ATP
vesicle associated enzyme at pH 7.3
Sus scrofa
1.28
-
ATP
vesicle associated enzyme at pH 9.9
Sus scrofa
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
human erythrocyte membrane
Sus scrofa
16020
-
soluble
two different localisations found
Sus scrofa
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
61000
-
SDS-PAGE, soluble enzyme
Sus scrofa
127000
-
SDS-PAGE, sedimentable enzyme
Sus scrofa
Purification (Commentary) (protein specific)
Commentary
Organism
-
Sus scrofa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + H2O
-
210227
Sus scrofa
AMP + diphosphate
-
210227
Sus scrofa
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.9
-
particular enzymes
Sus scrofa
Other publictions for EC 3.6.1.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
668024
Martins
Inhibition of the ecto-ATPdiph ...
Schistosoma mansoni
Biosci. Rep.
20
369-381
2000
-
-
-
-
-
-
10
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
10
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726055
Rashid
Characterization of a RecA/RAD ...
Thermococcus kodakarensis
Nucleic Acids Res.
25
719-726
1997
-
-
1
-
-
-
-
-
-
1
2
-
-
4
-
-
1
-
-
-
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-
1
1
1
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1
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1
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-
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-
-
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-
1
2
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-
1
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
210227
Masuda
A unique ectonucleotide pyroph ...
Sus scrofa
J. Clin. Invest.
95
699-704
1995
-
-
-
-
-
-
-
2
2
-
2
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
2
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
210226
Capito
Presence of ATP-pyrophosphohyd ...
Mus musculus
Diabetes
35
1096-1100
1986
6
-
-
-
-
-
1
3
-
7
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
1
-
3
-
7
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
210225
Torp-Pedersen
Studies on a Ca2+-dependent nu ...
Rattus norvegicus
Biochim. Biophys. Acta
571
94-104
1979
-
-
-
-
-
-
2
2
1
4
1
2
-
2
-
-
-
-
-
3
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
2
1
4
1
2
-
-
-
-
-
3
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
210224
Flodgaard
A calcium ion-dependent adenos ...
Rattus norvegicus
Biochem. J.
171
817-820
1978
-
-
-
-
-
-
-
-
1
4
-
1
-
2
-
-
1
-
-
2
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
4
-
1
-
-
-
1
-
2
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
210223
Kawamura
Change in ATP-pyrophosphohydro ...
Physarum polycephalum
Biochim. Biophys. Acta
421
195-202
1976
-
-
-
-
-
-
2
-
-
-
-
1
-
2
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
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-
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-
-
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-
-
2
-
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-
-
1
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1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
210222
Kawamura
A calcium ion-dependent ATP py ...
Physarum polycephalum
Biochim. Biophys. Acta
397
207-219
1975
-
-
-
-
-
-
1
1
-
8
2
3
-
3
-
-
-
-
-
1
-
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
8
2
3
-
-
-
-
-
1
-
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
210221
Heppel
Mechanism of enzymatic hydroly ...
Bos taurus, Crotalus adamanteus, Oryctolagus cuniculus, Rattus norvegicus
J. Biol. Chem.
202
217-226
1953
-
-
-
-
-
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-
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4
-
5
-
4
-
-
1
-
-
2
-
-
5
-
-
-
1
-
1
2
-
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-
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-
-
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-
-
-
-
-
-
-
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-
4
-
5
-
-
-
1
-
2
-
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5
-
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-
1
-
1
2
-
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-
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-