BRENDA - Enzyme Database
show all sequences of 3.6.1.8

Studies on a Ca2+-dependent nucleoside triphosphate pyrophosphohydrolase in rat liver plasma membranes

Torp-Pedersen, C.; Flodgaard, H.; Saermark, T.; Biochim. Biophys. Acta 571, 94-104 (1979)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
GMP
competitive inhibition with Ki: 0.001 mM
Rattus norvegicus
UDPGlc
very weak
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00032
-
ATP
with 0.1 mM free Ca2+, pH 8
Rattus norvegicus
0.0022
-
GTP
with 0.1 mM free Ca2+, pH 8
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
human erythrocyte membrane
Rattus norvegicus
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ba2+
weak activation
Rattus norvegicus
Ca2+
required for activity
Rattus norvegicus
Mg2+
weak activation
Rattus norvegicus
Sr2+
weak activation
Rattus norvegicus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
120000
-
-
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + H2O
Rattus norvegicus
-
AMP + diphosphate
-
Rattus norvegicus
?
CTP + H2O
Rattus norvegicus
-
CMP + diphosphate
-
Rattus norvegicus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
additional information
18 tissues examined
Rattus norvegicus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + H2O
-
210225
Rattus norvegicus
AMP + diphosphate
-
210225
Rattus norvegicus
?
CTP + H2O
-
210225
Rattus norvegicus
CMP + diphosphate
-
210225
Rattus norvegicus
?
dATP + H2O
-
210225
Rattus norvegicus
dAMP + diphosphate
-
210225
Rattus norvegicus
?
GTP + H2O
-
210225
Rattus norvegicus
GMP + diphosphate
-
-
-
-
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.001
-
GMP
competitive inhibition
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
GMP
competitive inhibition with Ki: 0.001 mM
Rattus norvegicus
UDPGlc
very weak
Rattus norvegicus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.001
-
GMP
competitive inhibition
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00032
-
ATP
with 0.1 mM free Ca2+, pH 8
Rattus norvegicus
0.0022
-
GTP
with 0.1 mM free Ca2+, pH 8
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
human erythrocyte membrane
Rattus norvegicus
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ba2+
weak activation
Rattus norvegicus
Ca2+
required for activity
Rattus norvegicus
Mg2+
weak activation
Rattus norvegicus
Sr2+
weak activation
Rattus norvegicus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
120000
-
-
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + H2O
Rattus norvegicus
-
AMP + diphosphate
-
Rattus norvegicus
?
CTP + H2O
Rattus norvegicus
-
CMP + diphosphate
-
Rattus norvegicus
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
additional information
18 tissues examined
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + H2O
-
210225
Rattus norvegicus
AMP + diphosphate
-
210225
Rattus norvegicus
?
CTP + H2O
-
210225
Rattus norvegicus
CMP + diphosphate
-
210225
Rattus norvegicus
?
dATP + H2O
-
210225
Rattus norvegicus
dAMP + diphosphate
-
210225
Rattus norvegicus
?
GTP + H2O
-
210225
Rattus norvegicus
GMP + diphosphate
-
-
-
-
Other publictions for EC 3.6.1.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
668024
Martins
Inhibition of the ecto-ATPdiph ...
Schistosoma mansoni
Biosci. Rep.
20
369-381
2000
-
-
-
-
-
-
10
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
10
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726055
Rashid
Characterization of a RecA/RAD ...
Thermococcus kodakarensis
Nucleic Acids Res.
25
719-726
1997
-
-
1
-
-
-
-
-
-
1
2
-
-
4
-
-
1
-
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
1
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
210227
Masuda
A unique ectonucleotide pyroph ...
Sus scrofa
J. Clin. Invest.
95
699-704
1995
-
-
-
-
-
-
-
2
2
-
2
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
2
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
210226
Capito
Presence of ATP-pyrophosphohyd ...
Mus musculus
Diabetes
35
1096-1100
1986
6
-
-
-
-
-
1
3
-
7
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
1
-
3
-
7
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
210225
Torp-Pedersen
Studies on a Ca2+-dependent nu ...
Rattus norvegicus
Biochim. Biophys. Acta
571
94-104
1979
-
-
-
-
-
-
2
2
1
4
1
2
-
2
-
-
-
-
-
3
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
2
1
4
1
2
-
-
-
-
-
3
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
210224
Flodgaard
A calcium ion-dependent adenos ...
Rattus norvegicus
Biochem. J.
171
817-820
1978
-
-
-
-
-
-
-
-
1
4
-
1
-
2
-
-
1
-
-
2
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
4
-
1
-
-
-
1
-
2
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
210223
Kawamura
Change in ATP-pyrophosphohydro ...
Physarum polycephalum
Biochim. Biophys. Acta
421
195-202
1976
-
-
-
-
-
-
2
-
-
-
-
1
-
2
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
210222
Kawamura
A calcium ion-dependent ATP py ...
Physarum polycephalum
Biochim. Biophys. Acta
397
207-219
1975
-
-
-
-
-
-
1
1
-
8
2
3
-
3
-
-
-
-
-
1
-
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
8
2
3
-
-
-
-
-
1
-
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
210221
Heppel
Mechanism of enzymatic hydroly ...
Bos taurus, Crotalus adamanteus, Oryctolagus cuniculus, Rattus norvegicus
J. Biol. Chem.
202
217-226
1953
-
-
-
-
-
-
-
-
-
4
-
5
-
4
-
-
1
-
-
2
-
-
5
-
-
-
1
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
5
-
-
-
1
-
2
-
-
5
-
-
-
1
-
1
2
-
-
-
-
-
-
-
-