BRENDA - Enzyme Database
show all sequences of 3.6.1.8

A calcium ion-dependent ATP pyrophosphohydrolase in Physarum polycephalum

Kawamura, M.; Nagano, K.; Biochim. Biophys. Acta 397, 207-219 (1975)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Caffeine
25% inhibition at 3 mM
Physarum polycephalum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2
-
ATP
-
Physarum polycephalum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ba2+
no effect
Physarum polycephalum
Ca2+
required for activity
Physarum polycephalum
Cd2+
no effect
Physarum polycephalum
Co2+
no effect
Physarum polycephalum
Cu2+
no effect
Physarum polycephalum
Mg2+
weak activation
Physarum polycephalum
Mn2+
no effect
Physarum polycephalum
Sr2+
partial substitution for Ca2+
Physarum polycephalum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
56000
-
SDS-PAGE
Physarum polycephalum
240000
-
gel filtration
Physarum polycephalum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + H2O
Physarum polycephalum
-
AMP + diphosphate
-
Physarum polycephalum
?
GTP + H2O
Physarum polycephalum
-
GMP + diphosphate
-
Physarum polycephalum
?
ITP + H2O
Physarum polycephalum
-
IMP + diphosphate
-
Physarum polycephalum
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Physarum polycephalum
-
acellular slime mold
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
plasmodium
-
Physarum polycephalum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + H2O
-
210222
Physarum polycephalum
AMP + diphosphate
-
210222
Physarum polycephalum
?
beta,gamma-methylene ATP + H2O
-
210222
Physarum polycephalum
AMP + methylene diphosphate
-
210222
Physarum polycephalum
?
GTP + H2O
-
210222
Physarum polycephalum
GMP + diphosphate
-
210222
Physarum polycephalum
?
ITP + H2O
-
210222
Physarum polycephalum
IMP + diphosphate
-
210222
Physarum polycephalum
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 56000, SDS-PAGE and gel filtration
Physarum polycephalum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
broad optimum range
Physarum polycephalum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Caffeine
25% inhibition at 3 mM
Physarum polycephalum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2
-
ATP
-
Physarum polycephalum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ba2+
no effect
Physarum polycephalum
Ca2+
required for activity
Physarum polycephalum
Cd2+
no effect
Physarum polycephalum
Co2+
no effect
Physarum polycephalum
Cu2+
no effect
Physarum polycephalum
Mg2+
weak activation
Physarum polycephalum
Mn2+
no effect
Physarum polycephalum
Sr2+
partial substitution for Ca2+
Physarum polycephalum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
56000
-
SDS-PAGE
Physarum polycephalum
240000
-
gel filtration
Physarum polycephalum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + H2O
Physarum polycephalum
-
AMP + diphosphate
-
Physarum polycephalum
?
GTP + H2O
Physarum polycephalum
-
GMP + diphosphate
-
Physarum polycephalum
?
ITP + H2O
Physarum polycephalum
-
IMP + diphosphate
-
Physarum polycephalum
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
plasmodium
-
Physarum polycephalum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + H2O
-
210222
Physarum polycephalum
AMP + diphosphate
-
210222
Physarum polycephalum
?
beta,gamma-methylene ATP + H2O
-
210222
Physarum polycephalum
AMP + methylene diphosphate
-
210222
Physarum polycephalum
?
GTP + H2O
-
210222
Physarum polycephalum
GMP + diphosphate
-
210222
Physarum polycephalum
?
ITP + H2O
-
210222
Physarum polycephalum
IMP + diphosphate
-
210222
Physarum polycephalum
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 56000, SDS-PAGE and gel filtration
Physarum polycephalum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
broad optimum range
Physarum polycephalum
Other publictions for EC 3.6.1.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
668024
Martins
Inhibition of the ecto-ATPdiph ...
Schistosoma mansoni
Biosci. Rep.
20
369-381
2000
-
-
-
-
-
-
10
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
10
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726055
Rashid
Characterization of a RecA/RAD ...
Thermococcus kodakarensis
Nucleic Acids Res.
25
719-726
1997
-
-
1
-
-
-
-
-
-
1
2
-
-
4
-
-
1
-
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
1
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
210227
Masuda
A unique ectonucleotide pyroph ...
Sus scrofa
J. Clin. Invest.
95
699-704
1995
-
-
-
-
-
-
-
2
2
-
2
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
2
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
210226
Capito
Presence of ATP-pyrophosphohyd ...
Mus musculus
Diabetes
35
1096-1100
1986
6
-
-
-
-
-
1
3
-
7
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
1
-
3
-
7
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
210225
Torp-Pedersen
Studies on a Ca2+-dependent nu ...
Rattus norvegicus
Biochim. Biophys. Acta
571
94-104
1979
-
-
-
-
-
-
2
2
1
4
1
2
-
2
-
-
-
-
-
3
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
2
1
4
1
2
-
-
-
-
-
3
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
210224
Flodgaard
A calcium ion-dependent adenos ...
Rattus norvegicus
Biochem. J.
171
817-820
1978
-
-
-
-
-
-
-
-
1
4
-
1
-
2
-
-
1
-
-
2
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
4
-
1
-
-
-
1
-
2
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
210223
Kawamura
Change in ATP-pyrophosphohydro ...
Physarum polycephalum
Biochim. Biophys. Acta
421
195-202
1976
-
-
-
-
-
-
2
-
-
-
-
1
-
2
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
210222
Kawamura
A calcium ion-dependent ATP py ...
Physarum polycephalum
Biochim. Biophys. Acta
397
207-219
1975
-
-
-
-
-
-
1
1
-
8
2
3
-
3
-
-
-
-
-
1
-
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
8
2
3
-
-
-
-
-
1
-
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
210221
Heppel
Mechanism of enzymatic hydroly ...
Bos taurus, Crotalus adamanteus, Oryctolagus cuniculus, Rattus norvegicus
J. Biol. Chem.
202
217-226
1953
-
-
-
-
-
-
-
-
-
4
-
5
-
4
-
-
1
-
-
2
-
-
5
-
-
-
1
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
5
-
-
-
1
-
2
-
-
5
-
-
-
1
-
1
2
-
-
-
-
-
-
-
-