BRENDA - Enzyme Database
show all sequences of 3.6.1.8

Mechanism of enzymatic hydrolysis of adenosinetriphosphate

Heppel, L.A.; Hilmoe, R.J.; J. Biol. Chem. 202, 217-226 (1953)

Data extracted from this reference:

Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
no effect
Bos taurus
Ca2+
-
Rattus norvegicus
Mg2+
no effect
Bos taurus
Mg2+
-
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + H2O
Rattus norvegicus
-
AMP + diphosphate
-
Rattus norvegicus
?
ATP + H2O
Bos taurus
-
AMP + diphosphate
-
Bos taurus
?
ATP + H2O
Oryctolagus cuniculus
-
AMP + diphosphate
-
Oryctolagus cuniculus
?
ATP + H2O
Crotalus adamanteus
-
AMP + diphosphate
-
Crotalus adamanteus
?
ITP + H2O
Crotalus adamanteus
-
IMP + diphosphate
-
Crotalus adamanteus
?
Organism
Organism
UniProt
Commentary
Textmining
Bos taurus
-
-
-
Crotalus adamanteus
-
snake
-
Oryctolagus cuniculus
-
-
-
Rattus norvegicus
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
partial purification
Bos taurus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
-
Rattus norvegicus
-
plasma
-
Bos taurus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ATP + H2O
-
210221
Rattus norvegicus
AMP + diphosphate
-
210221
Rattus norvegicus
?
ATP + H2O
-
210221
Bos taurus
AMP + diphosphate
-
210221
Bos taurus
?
ATP + H2O
-
210221
Oryctolagus cuniculus
AMP + diphosphate
-
210221
Oryctolagus cuniculus
?
ATP + H2O
-
210221
Crotalus adamanteus
AMP + diphosphate
-
210221
Crotalus adamanteus
?
ITP + H2O
-
210221
Crotalus adamanteus
IMP + diphosphate
-
210221
Crotalus adamanteus
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
30 min at 60°C causes no loss of activity
Bos taurus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.4
9
-
Bos taurus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
10
20% activity at pH 6, 50% activity at pH 10
Bos taurus
7.4
8.8
much higher activity at pH 8.8
Crotalus adamanteus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
no effect
Bos taurus
Ca2+
-
Rattus norvegicus
Mg2+
no effect
Bos taurus
Mg2+
-
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + H2O
Rattus norvegicus
-
AMP + diphosphate
-
Rattus norvegicus
?
ATP + H2O
Bos taurus
-
AMP + diphosphate
-
Bos taurus
?
ATP + H2O
Oryctolagus cuniculus
-
AMP + diphosphate
-
Oryctolagus cuniculus
?
ATP + H2O
Crotalus adamanteus
-
AMP + diphosphate
-
Crotalus adamanteus
?
ITP + H2O
Crotalus adamanteus
-
IMP + diphosphate
-
Crotalus adamanteus
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial purification
Bos taurus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
-
Rattus norvegicus
-
plasma
-
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ATP + H2O
-
210221
Rattus norvegicus
AMP + diphosphate
-
210221
Rattus norvegicus
?
ATP + H2O
-
210221
Bos taurus
AMP + diphosphate
-
210221
Bos taurus
?
ATP + H2O
-
210221
Oryctolagus cuniculus
AMP + diphosphate
-
210221
Oryctolagus cuniculus
?
ATP + H2O
-
210221
Crotalus adamanteus
AMP + diphosphate
-
210221
Crotalus adamanteus
?
ITP + H2O
-
210221
Crotalus adamanteus
IMP + diphosphate
-
210221
Crotalus adamanteus
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
30 min at 60°C causes no loss of activity
Bos taurus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.4
9
-
Bos taurus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
10
20% activity at pH 6, 50% activity at pH 10
Bos taurus
7.4
8.8
much higher activity at pH 8.8
Crotalus adamanteus
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
668024
Martins
Inhibition of the ecto-ATPdiph ...
Schistosoma mansoni
Biosci. Rep.
20
369-381
2000
-
-
-
-
-
-
10
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
10
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726055
Rashid
Characterization of a RecA/RAD ...
Thermococcus kodakarensis
Nucleic Acids Res.
25
719-726
1997
-
-
1
-
-
-
-
-
-
1
2
-
-
4
-
-
1
-
-
-
-
-
1
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
1
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
210227
Masuda
A unique ectonucleotide pyroph ...
Sus scrofa
J. Clin. Invest.
95
699-704
1995
-
-
-
-
-
-
-
2
2
-
2
-
-
3
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
2
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
210226
Capito
Presence of ATP-pyrophosphohyd ...
Mus musculus
Diabetes
35
1096-1100
1986
6
-
-
-
-
-
1
3
-
7
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
1
-
3
-
7
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
210225
Torp-Pedersen
Studies on a Ca2+-dependent nu ...
Rattus norvegicus
Biochim. Biophys. Acta
571
94-104
1979
-
-
-
-
-
-
2
2
1
4
1
2
-
2
-
-
-
-
-
3
-
-
4
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
2
1
4
1
2
-
-
-
-
-
3
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
210224
Flodgaard
A calcium ion-dependent adenos ...
Rattus norvegicus
Biochem. J.
171
817-820
1978
-
-
-
-
-
-
-
-
1
4
-
1
-
2
-
-
1
-
-
2
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
4
-
1
-
-
-
1
-
2
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
210223
Kawamura
Change in ATP-pyrophosphohydro ...
Physarum polycephalum
Biochim. Biophys. Acta
421
195-202
1976
-
-
-
-
-
-
2
-
-
-
-
1
-
2
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
210222
Kawamura
A calcium ion-dependent ATP py ...
Physarum polycephalum
Biochim. Biophys. Acta
397
207-219
1975
-
-
-
-
-
-
1
1
-
8
2
3
-
3
-
-
-
-
-
1
-
-
4
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
8
2
3
-
-
-
-
-
1
-
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
210221
Heppel
Mechanism of enzymatic hydroly ...
Bos taurus, Crotalus adamanteus, Oryctolagus cuniculus, Rattus norvegicus
J. Biol. Chem.
202
217-226
1953
-
-
-
-
-
-
-
-
-
4
-
5
-
4
-
-
1
-
-
2
-
-
5
-
-
-
-
1
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
5
-
-
-
1
-
2
-
-
5
-
-
-
1
-
1
2
-
-
-
-
-
-
-
-