Cloned (Comment) | Organism |
---|---|
expression of TT0497 in Escherichia coli strain BL21(DE3) | Thermus thermophilus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant TT0497, 0.0005 ml of 2.9 mg/ml protein in 20 mM Tris-HCl pH 8.0, 0.2 M NaCl is mixed with 0.0005 ml of precipitant solution containing 27.5% w/v PEG 4000 and 0.1 M MES, pH 6.9, X-ray diffraction structure determination and analysis at 1.3 A resolution, structural refinement based on the Fourier method, modelling, overview | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q5SKS6 | - |
- |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | Q5SKS6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant TT0497 from Escherichia coli strain BL21(DE3) by two steps of ion exchange chromatography, and gel filtration | Thermus thermophilus |
Subunits | Comment | Organism |
---|---|---|
More | structure modelling, multiple conformations of the side chains of amino-acid residues | Thermus thermophilus |
Synonyms | Comment | Organism |
---|---|---|
TT0497 | - |
Thermus thermophilus |