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Literature summary for 3.6.1.7 extracted from

  • Miyazono, K.I.; Kudo, N.; Tanokura, M.
    Cloning, purification, crystallization and preliminary crystallographic analysis of acylphosphatase from Pyrococcus horikoshii OT3 (2004), Acta Crystallogr. Sect. D, 60, 1135-1136.
    View publication on PubMed
Search for more literature for 3.6.1.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain Rosetta Pyrococcus horikoshii
expression in Escherichia coli Pyrococcus horikoshii

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapour diffusion method with potassium/sodium tartrate at pH 5.5 Pyrococcus horikoshii
sitting-drop vapour-diffusion method with postasium/sodium tartrate as the precipitant at pH 5.5. The crystals belong to space group P3(2)21, with approximate unit-cell parameters a = b = 86.7 A, c = 75.4 A and two monomers in the asymmetric unit Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii
-
 strain OT3
-
Pyrococcus horikoshii OT-3
-
 strain OT3
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus horikoshii
ammonium sulfate fractionation and cation exchange chromatography Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3-diphosphoglycerate + H2O
-
 Pyrococcus horikoshii 3-phosphoglycerate + phosphate
-
 ?
1,3-diphosphoglycerate + H2O
-
 Pyrococcus horikoshii OT-3 3-phosphoglycerate + phosphate
-
 ?
acetyl phosphate + H2O
-
 Pyrococcus horikoshii acetate + phosphate
-
 ?
acetyl phosphate + H2O
-
 Pyrococcus horikoshii OT-3 acetate + phosphate
-
 ?
benzoyl phosphate + H2O
-
 Pyrococcus horikoshii benzoate + phosphate
-
 ?
benzoyl phosphate + H2O
-
 Pyrococcus horikoshii OT-3 benzoate + phosphate
-
 ?
beta-aspartyl phosphate + H2O
-
 Pyrococcus horikoshii aspartate + phosphate
-
 ?
beta-aspartyl phosphate + H2O
-
 Pyrococcus horikoshii OT-3 aspartate + phosphate
-
 ?
carbamoyl phosphate + H2O
-
 Pyrococcus horikoshii carbamate + phosphate
-
 ?
carbamoyl phosphate + H2O
-
 Pyrococcus horikoshii OT-3 carbamate + phosphate
-
 ?
succinyl phosphate + H2O
-
 Pyrococcus horikoshii succinate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
ACP
-
 Pyrococcus horikoshii