Cloned (Comment) | Organism |
---|---|
wild-type and Cys21 mutants: C21A and C21S, expressed in Escherichia coli strain BL21 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C21A | reduced specific activity, 60% compared with wild-type enzyme, kinetic and structural properties similar to those of wild-type recombinant enzyme, urea and thermal stabilities reduced: Cys21 possible involved in stabilization of enzyme active-site conformation, involved in enzyme structure stabilization, not involved in substrate binding | Homo sapiens |
C21S | reduced specific activity, 85% compared with wild-type enzyme, kinetic and structural properties similar to those of wild-type recombinant enzyme, urea and thermal stabilities reduced: Cys21 possible involved in stabilization of enzyme active-site conformation, involved in enzyme structure stabilization, not involved in substrate binding | Homo sapiens |
General Stability | Organism |
---|---|
muscular enzyme, sensitive to urea: urea denaturation | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | inhibition partially reactivated in the absence of Hg2+, mutant enzymes regained 65-85% of original activity, wild-type enzyme regained less than 50% | Homo sapiens | |
phosphate | phosphate, competitive inhibition, muscular isoenzyme, wild-type, Ki: 0.9 mM, C21A Ki: 0.9 mM, C21S Ki: 0.7 mM | Homo sapiens | |
Urea | 8 M: 100% inhibition; wild-type and mutants, urea inactivation studies | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic data | Homo sapiens | |
0.4 | - |
benzoyl phosphate | C21A | Homo sapiens | |
0.5 | - |
benzoyl phosphate | wild-type, muscle | Homo sapiens | |
0.8 | - |
benzoyl phosphate | C21S | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
human | - |
Homo sapiens | - |
wild-type and Cys21 mutants: C21A and C21S | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and Cys21 mutants: C21A and C21S | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an acylphosphate + H2O = a carboxylate + phosphate | catalytic mechanism | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
renaturation after treatment with urea | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4300 | - |
C21A | Homo sapiens |
5900 | - |
C21S | Homo sapiens |
7000 | - |
wild-type, muscle | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3-diphosphoglycerate + H2O | - |
Homo sapiens | 3-phosphoglycerate + phosphate | - |
? | |
acylphosphate + H2O | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Homo sapiens | carboxylate + phosphate | - |
? | |
benzoyl phosphate + H2O | - |
Homo sapiens | benzoate + phosphate | - |
? | |
carbamoyl phosphate + H2O | - |
Homo sapiens | carbamate + phosphate | - |
? | |
additional information | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Homo sapiens | ? | - |
? | |
additional information | hydrolyzes the beta-aspartylphosphate formed during the action of membrane pumps | Homo sapiens | ? | - |
? | |
succinoyl phosphate + H2O | - |
Homo sapiens | succinate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | two isoenzymatic forms: one prevalent in skeletal and cardiac muscle named muscle type, the other in red blood cells named erythrocyte or common type, even if both isoforms are expressed in all tissues | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermal inactivation studies | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
muscle enzyme is very basic protein | Homo sapiens |
4.5 | 5.5 | C21A | Homo sapiens |
4.7 | 5.6 | C21S | Homo sapiens |
4.8 | 5.8 | wild-type, muscle | Homo sapiens |