Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3-diphosphoglycerate | erythrocyte isoenzyme, competitive inhibition, Ki: 0.24 mM | Bos taurus | |
3-phosphoglycerate | erythrocyte isoenzyme, competitive inhibition, Ki: 1.70 mM | Bos taurus | |
Cl- | erythrocyte isoenzyme, competitive inhibition, Ki: 18.0 mM | Bos taurus | |
phosphate | erythrocyte isoenzyme, competitive inhibition, Ki: 0.41 mM | Bos taurus | |
phosphoenolpyruvate | erythrocyte isoenzyme, competitive inhibition, Ki: 0.21 mM | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic studies | Bos taurus | |
additional information | - |
additional information | kinetic characterization, kinetic data | Bos taurus | |
0.12 | - |
benzoyl phosphate | erythrocyte enzyme | Bos taurus | |
4.23 | - |
acetyl phosphate | erythrocyte enzyme | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Bos taurus | 5737 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bos taurus | since acylphosphatase hydrolyzes the high-energy phosphorylated compounds its action is probably involved in the regulation of cell energy metabolism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
bovine | - |
Bos taurus | - |
two isoenzymes: muscular isoenzyme, IM, and erythrocyte isoenzyme, IE | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | erythrocyte isoenzyme, NH2-terminus is acetylated | Bos taurus |
Purification (Comment) | Organism |
---|---|
erythrocyte form isoenzyme | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | erythrocyte isoenzyme | Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6800 | - |
erythrocyte form isoenzyme | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(Ca2+-Mg2+)-ATPase phosphorylated intermediate + H2O | - |
Bos taurus | ? | - |
? | |
1,3-diphosphoglycerate + H2O | - |
Bos taurus | 3-phosphoglycerate + phosphate | - |
? | |
acetyl phosphate + H2O | - |
Bos taurus | acetate + phosphate | - |
? | |
acylphosphate + H2O | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Bos taurus | carboxylate + phosphate | - |
? | |
benzoyl phosphate + H2O | - |
Bos taurus | benzoate + phosphate | - |
? | |
carbamoyl phosphate + H2O | - |
Bos taurus | carbamate + phosphate | - |
? | |
additional information | since acylphosphatase hydrolyzes the high-energy phosphorylated compounds its action is probably involved in the regulation of cell energy metabolism | Bos taurus | ? | - |
? | |
Na+/K+-ATPase phosphoenzyme intermediate + H2O | - |
Bos taurus | ? | - |
? | |
succinoyl phosphate + H2O | - |
Bos taurus | succinate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | two isoenzymes in all tissues, organs, and cell types checked: 1. muscular isoenzyme, IM, and 2. erythrocyte isoenzyme, IE, on the basis of the cell-type in which they are mostly represented and from which they are purified for the first time | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
117 | - |
benzoyl phosphate | - |
Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | - |
erythrocyte isoenzyme | Bos taurus |
5.3 | - |
assay at | Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
erythrocyte isoenzyme, retains activity over a broad range of pH values | Bos taurus |