Literature summary for 3.6.1.7 extracted from

Stefani, M.; Degl'Innocenti, D.; Berti, A.; Cappugi, G.; Manao, G.; Camici, G.; Ramponi, G.
Purification and characterization of acylphosphatase erythrocyte isoenzyme from turkey muscle (1990), J. Protein Chem., 9, 633-640.

Search for more literature for 3.6.1.7

General Stability

General Stability	Organism
enzyme very sensitive to 8 M urea	Meleagris gallopavo

Inhibitors

Inhibitors	Comment	Organism	Structure
Cl-	muscular isoenzyme, Ki: 40.0 mM, erythrocyte isoenzyme, Ki: 31.0 mM	Meleagris gallopavo
Phenylglyoxal	6 mM: inhibition, inhibition partially removed by 35 mM phosphate	Meleagris gallopavo
phosphate	muscular isoenzyme, Ki: 2.8 mM, erythrocyte isoenzyme, Ki: 0.68 mM	Meleagris gallopavo
Urea	8 M: 100% inhibition; 8 M: 100% reversibel inhibition, 60% reactivation in the absence of urea	Meleagris gallopavo

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kinetic data	Meleagris gallopavo
0.18	-	benzoyl phosphate	erythrocyte isoenzyme	Meleagris gallopavo
1	-	benzoyl phosphate	muscular isoenzyme	Meleagris gallopavo

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
12750	-	SDS-PAGE	Meleagris gallopavo
12750	-	erythrocyte isoenzyme	Meleagris gallopavo
13400	-	muscular isoenzyme	Meleagris gallopavo

Organism

Organism	UniProt	Comment	Textmining
Meleagris gallopavo	-	turkey	-
Meleagris gallopavo	-	two isoenzymes: muscular isoenzyme, IM, and erythrocyte isoenzyme, IE	-

Purification (Commentary)

Purification (Comment)	Organism
muscle, erythrocyte isoenzyme	Meleagris gallopavo

Reaction

Reaction	Comment	Organism	Reaction ID
an acylphosphate + H2O = a carboxylate + phosphate	thermodynamic data	Meleagris gallopavo	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
muscle	skeletal muscle	Meleagris gallopavo	-
muscle	erythrocyte isoenzyme	Meleagris gallopavo	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3500	-	muscular isoenzyme	Meleagris gallopavo
9000	-	erythrocyte isoenzyme	Meleagris gallopavo

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1,3-diphosphoglycerate + H2O	-	Meleagris gallopavo	3-phosphoglycerate + phosphate	-	?
acylphosphate + H2O	specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates	Meleagris gallopavo	carboxylate + phosphate	-	?
benzoyl phosphate + H2O	-	Meleagris gallopavo	benzoate + phosphate	-	?

Subunits

Subunits	Comment	Organism
monomer	erythrocyte isoenzyme, 1 * 12750, SDS-PAGE	Meleagris gallopavo

Synonyms

Synonyms	Comment	Organism
More	two isoenzymes in all tissues, organs, and cell types checked: 1. muscular isoenzyme, IM, and 2. erythrocyte isoenzyme, IE, on the basis of the cell-type in which they are mostly represented and from which they are purified for the first time	Meleagris gallopavo

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	enzyme is thermostable	Meleagris gallopavo
additional information	-	enzyme is thermostable, thermostability is pH-dependent, turkey enzyme is less unstable at higher pH values than horse enzyme	Meleagris gallopavo

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
additional information	-	erythrocyte enzyme is a basic protein	Meleagris gallopavo
5.3	-	muscular and erythrocyte isoenzyme	Meleagris gallopavo

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)