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Literature summary for 3.6.1.7 extracted from
- 1,3-Diphosphoglycerate phosphatase (1975), Methods Enzymol., 42C, 409-426.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Oryctolagus cuniculus |
General Stability
| General Stability | Organism |
|---|---|
| brain enzyme, unusually stable to prolonged storage, lyophilization and to acid treatment | Bos taurus |
| enzyme is very stable | Gallus gallus |
| enzyme is very stable | Sus scrofa |
| enzyme is very stable | Bos taurus |
| enzyme is very stable | Oryctolagus cuniculus |
| enzyme is very stable | Equus caballus |
| liver enzyme, stable to acid treatment | Equus caballus |
| muscle enzyme, stable to various denaturating agents | Equus caballus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,3-diphosphoglycerate | - |
Bos taurus |  |
| 2,3-diphosphoglycerate | - |
Homo sapiens | |
| 2,3-diphosphoglycerate | heart enzyme, 2 mM: 12% inhibition, substrate: 1,3-diphosphoglycerate | Sus scrofa | |
| 3-phosphoglycerate | - |
Bos taurus | |
| 3-phosphoglycerate | heart enzyme, 5 mM: 8% inhibition, substrate: 1,3-diphosphoglycerate | Sus scrofa | |
| ATP | noncompetitive inhibition, Ki: 0.57 mM | Equus caballus | |
| ATP | erythrocyte enzyme, competitive inhibition, Ki: 4.4 mM | Homo sapiens | |
| ATP | heart enzyme, 5 mM: 11% inhibition, substrate: 1,3-diphosphoglycerate | Sus scrofa | |
| benzyl phosphate | moderate competitive inhibition, Ki: 11 mM | Equus caballus | |
| Carbamoyl phosphate | erythrocyte enzyme, competitive inhibition, Ki: 6.9 mM | Homo sapiens | |
| fructose 1,6-diphosphate | noncompetitive inhibition, Ki: 3.2 mM | Equus caballus | |
| iodoacetate | brain enzyme, 4 mM: no inhibition | Bos taurus | |
| iodoacetate | heart enzyme, 10 mM: very low, less than 5%, inhibition | Sus scrofa | |
| methyl phosphate | moderate competitive inhibition, Ki: 3.5 mM | Equus caballus | |
| additional information | brain enzyme, no inhibition by 0.04 mM HgCl2 and 4 mM iodoacetate | Bos taurus | |
| additional information | no inhibition by adenosine monophosphate, phosphoethanolamine and alpha-glycerophosphate | Equus caballus | |
| additional information | - |
Gallus gallus | |
| additional information | heart enzyme, no inhibition by 2.0 mM HgCl2 | Sus scrofa | |
| Orotic acid | liver enzyme, noncompetitive inhibition | Equus caballus | |
| p-chloromercuribenzoate | heart enzyme, 1 mM: very low, less than 5%, inhibition | Sus scrofa | |
| phosphate | - |
Bos taurus | |
| phosphate | phosphate: high competitive inhibition, mechanism of inhibition | Equus caballus | |
| phosphate | eryhrocyte enzyme, competitive inhibition, Ki: 3.4 mM | Homo sapiens | |
| phosphate | heart enzyme, 5 mM: 37% inhibition, substrate: 1,3-diphosphoglycerate; heart enzyme, competitive inhibition | Sus scrofa | |
| Phosphorylated compounds | - |
Equus caballus | |
| pyridoxal 5'-phosphate | muscle enzyme, pH-dependent competitive, reversible inhibition, Ki: 0.32 mM | Equus caballus | |
| sulfate | Si, heart enzyme, competitive inhibition | Sus scrofa | |
| Urea | 8 M: 100% inhibition | Bos taurus | |
| Urea | 8 M: 100% inhibition | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
1,3-diphosphoglycerate | erythrocyte enzyme | Homo sapiens | |
| 1.6 | - |
1,3-diphosphoglycerate | muscle | Equus caballus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | brain enzyme, 15 mM: activation, substrate: Tris salt of acetyl phosphate | Bos taurus | |
| MgCl2 | - |
Homo sapiens | |
| MgCl2 | brain enzyme, 5 mM: activation, substrate: Tris salt of acetyl phosphate | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 8300 | - |
liver, gel filtration | Equus caballus |
| 9400 | - |
muscle, low speed sedimentation equilibrium, Archibald method, and gel filtration | Equus caballus |
| 11100 | - |
heart, gel filtration | Sus scrofa |
| 12000 | - |
- |
Gallus gallus |
| 12000 | - |
brain, gel filtration | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,3-diphosphoglycerate + H2O | Gallus gallus | possible role in preventing the intracellular accumulation of 1,3-diphosphoglycerate by catalyzing the hydrolysis of this substrate to 3-phosphoglycerate | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | Homo sapiens | possible role in preventing the intracellular accumulation of 1,3-diphosphoglycerate by catalyzing the hydrolysis of this substrate to 3-phosphoglycerate | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | Sus scrofa | possible role in preventing the intracellular accumulation of 1,3-diphosphoglycerate by catalyzing the hydrolysis of this substrate to 3-phosphoglycerate | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | Saccharomyces cerevisiae | possible role in preventing the intracellular accumulation of 1,3-diphosphoglycerate by catalyzing the hydrolysis of this substrate to 3-phosphoglycerate | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | Bos taurus | possible role in preventing the intracellular accumulation of 1,3-diphosphoglycerate by catalyzing the hydrolysis of this substrate to 3-phosphoglycerate | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | Oryctolagus cuniculus | possible role in preventing the intracellular accumulation of 1,3-diphosphoglycerate by catalyzing the hydrolysis of this substrate to 3-phosphoglycerate | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | Equus caballus | possible role in preventing the intracellular accumulation of 1,3-diphosphoglycerate by catalyzing the hydrolysis of this substrate to 3-phosphoglycerate | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | Gallus gallus | 1,3-diphosphoglycerate can acylate histones, particularly the lysine-rich ones, 1,3-diphosphoglycerate phosphatase can prevent this acylation | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | Homo sapiens | 1,3-diphosphoglycerate can acylate histones, particularly the lysine-rich ones, 1,3-diphosphoglycerate phosphatase can prevent this acylation | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | Sus scrofa | 1,3-diphosphoglycerate can acylate histones, particularly the lysine-rich ones, 1,3-diphosphoglycerate phosphatase can prevent this acylation | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | Saccharomyces cerevisiae | 1,3-diphosphoglycerate can acylate histones, particularly the lysine-rich ones, 1,3-diphosphoglycerate phosphatase can prevent this acylation | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | Bos taurus | 1,3-diphosphoglycerate can acylate histones, particularly the lysine-rich ones, 1,3-diphosphoglycerate phosphatase can prevent this acylation | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | Oryctolagus cuniculus | 1,3-diphosphoglycerate can acylate histones, particularly the lysine-rich ones, 1,3-diphosphoglycerate phosphatase can prevent this acylation | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | Equus caballus | 1,3-diphosphoglycerate can acylate histones, particularly the lysine-rich ones, 1,3-diphosphoglycerate phosphatase can prevent this acylation | 3-phosphoglycerate + phosphate | - |
? | |
| additional information | Gallus gallus | possible role in preventing the intracellular accumulation of 1,3-diphosphoglycerate by catalyzing the hydrolysis of this substrate to 3-phosphoglycerate | ? | - |
? | |
| additional information | Homo sapiens | possible role in preventing the intracellular accumulation of 1,3-diphosphoglycerate by catalyzing the hydrolysis of this substrate to 3-phosphoglycerate | ? | - |
? | |
| additional information | Sus scrofa | possible role in preventing the intracellular accumulation of 1,3-diphosphoglycerate by catalyzing the hydrolysis of this substrate to 3-phosphoglycerate | ? | - |
? | |
| additional information | Saccharomyces cerevisiae | possible role in preventing the intracellular accumulation of 1,3-diphosphoglycerate by catalyzing the hydrolysis of this substrate to 3-phosphoglycerate | ? | - |
? | |
| additional information | Bos taurus | possible role in preventing the intracellular accumulation of 1,3-diphosphoglycerate by catalyzing the hydrolysis of this substrate to 3-phosphoglycerate | ? | - |
? | |
| additional information | Oryctolagus cuniculus | possible role in preventing the intracellular accumulation of 1,3-diphosphoglycerate by catalyzing the hydrolysis of this substrate to 3-phosphoglycerate | ? | - |
? | |
| additional information | Equus caballus | possible role in preventing the intracellular accumulation of 1,3-diphosphoglycerate by catalyzing the hydrolysis of this substrate to 3-phosphoglycerate | ? | - |
? | |
| additional information | Equus caballus | enzyme could control acylation and carbamylation of proteins by regulation the levels of reactive acyl and carbamoyl phosphates | ? | - |
? | |
| additional information | Equus caballus | possible physiological role for acylphosphatase activity may be regulation of metabolic pathways involving 1,3-diphosphoglycerate and carbamoyl phosphate, e.g. glycolytic pathway and pyrimidine biosynthesis, by its ability to hydrolyze these substrates | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
bovine | - |
| Equus caballus | - |
- |
- |
| Gallus gallus | - |
- |
- |
| Homo sapiens | - |
human | - |
| Oryctolagus cuniculus | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oryctolagus cuniculus |
| brain | Bos taurus |
| erythrocyte isoenzyme | Homo sapiens |
| heart muscle | Sus scrofa |
| heart muscle | Bos taurus |
| muscle and liver | Equus caballus |
| partial | Saccharomyces cerevisiae |
| partially from breast muscle | Gallus gallus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Gallus gallus | - |
| brain | - |
Homo sapiens | - |
| brain | - |
Sus scrofa | - |
| brain | - |
Bos taurus | - |
| brain | - |
Equus caballus | - |
| erythrocyte | - |
Gallus gallus | - |
| erythrocyte | - |
Homo sapiens | - |
| erythrocyte | - |
Bos taurus | - |
| heart | - |
Gallus gallus | - |
| heart | - |
Homo sapiens | - |
| heart | - |
Sus scrofa | - |
| heart | - |
Bos taurus | - |
| heart | - |
Equus caballus | - |
| liver | - |
Gallus gallus | - |
| liver | - |
Homo sapiens | - |
| liver | - |
Equus caballus | - |
| muscle | - |
Gallus gallus | - |
| muscle | - |
Homo sapiens | - |
| muscle | - |
Sus scrofa | - |
| muscle | - |
Oryctolagus cuniculus | - |
| muscle | - |
Equus caballus | - |
| muscle | heart muscle | Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 12 | - |
erythrocyte | Homo sapiens |
| 600 | - |
heart | Sus scrofa |
| 1250 | - |
brain | Bos taurus |
| 2800 | - |
liver | Equus caballus |
| 3000 | - |
muscle | Equus caballus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C stable for at least 1 year | Bos taurus |
| -20°C, long-time stable | Sus scrofa |
| brain enzyme, unusually stable to prolonged storage | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,3-diphosphoglycerate + H2O | - |
Gallus gallus | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | - |
Homo sapiens | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | - |
Sus scrofa | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | - |
Saccharomyces cerevisiae | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | - |
Bos taurus | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | - |
Oryctolagus cuniculus | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | - |
Equus caballus | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | possible role in preventing the intracellular accumulation of 1,3-diphosphoglycerate by catalyzing the hydrolysis of this substrate to 3-phosphoglycerate | Gallus gallus | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | possible role in preventing the intracellular accumulation of 1,3-diphosphoglycerate by catalyzing the hydrolysis of this substrate to 3-phosphoglycerate | Homo sapiens | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | possible role in preventing the intracellular accumulation of 1,3-diphosphoglycerate by catalyzing the hydrolysis of this substrate to 3-phosphoglycerate | Sus scrofa | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | possible role in preventing the intracellular accumulation of 1,3-diphosphoglycerate by catalyzing the hydrolysis of this substrate to 3-phosphoglycerate | Saccharomyces cerevisiae | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | possible role in preventing the intracellular accumulation of 1,3-diphosphoglycerate by catalyzing the hydrolysis of this substrate to 3-phosphoglycerate | Bos taurus | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | possible role in preventing the intracellular accumulation of 1,3-diphosphoglycerate by catalyzing the hydrolysis of this substrate to 3-phosphoglycerate | Oryctolagus cuniculus | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | possible role in preventing the intracellular accumulation of 1,3-diphosphoglycerate by catalyzing the hydrolysis of this substrate to 3-phosphoglycerate | Equus caballus | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | 1,3-diphosphoglycerate can acylate histones, particularly the lysine-rich ones, 1,3-diphosphoglycerate phosphatase can prevent this acylation | Gallus gallus | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | 1,3-diphosphoglycerate can acylate histones, particularly the lysine-rich ones, 1,3-diphosphoglycerate phosphatase can prevent this acylation | Homo sapiens | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | 1,3-diphosphoglycerate can acylate histones, particularly the lysine-rich ones, 1,3-diphosphoglycerate phosphatase can prevent this acylation | Sus scrofa | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | 1,3-diphosphoglycerate can acylate histones, particularly the lysine-rich ones, 1,3-diphosphoglycerate phosphatase can prevent this acylation | Saccharomyces cerevisiae | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | 1,3-diphosphoglycerate can acylate histones, particularly the lysine-rich ones, 1,3-diphosphoglycerate phosphatase can prevent this acylation | Bos taurus | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | 1,3-diphosphoglycerate can acylate histones, particularly the lysine-rich ones, 1,3-diphosphoglycerate phosphatase can prevent this acylation | Oryctolagus cuniculus | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | 1,3-diphosphoglycerate can acylate histones, particularly the lysine-rich ones, 1,3-diphosphoglycerate phosphatase can prevent this acylation | Equus caballus | 3-phosphoglycerate + phosphate | - |
? | |
| acetyl phosphate + H2O | - |
Gallus gallus | acetate + phosphate | - |
? | |
| acetyl phosphate + H2O | - |
Homo sapiens | acetate + phosphate | - |
? | |
| acetyl phosphate + H2O | - |
Sus scrofa | acetate + phosphate | - |
? | |
| acetyl phosphate + H2O | - |
Bos taurus | acetate + phosphate | - |
? | |
| acetyl phosphate + H2O | - |
Oryctolagus cuniculus | acetate + phosphate | - |
? | |
| acetyl phosphate + H2O | - |
Equus caballus | acetate + phosphate | - |
? | |
| acylphosphate + H2O | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Gallus gallus | carboxylate + phosphate | - |
? | |
| acylphosphate + H2O | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Homo sapiens | carboxylate + phosphate | - |
? | |
| acylphosphate + H2O | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Sus scrofa | carboxylate + phosphate | - |
? | |
| acylphosphate + H2O | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Saccharomyces cerevisiae | carboxylate + phosphate | - |
? | |
| acylphosphate + H2O | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Bos taurus | carboxylate + phosphate | - |
? | |
| acylphosphate + H2O | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Oryctolagus cuniculus | carboxylate + phosphate | - |
? | |
| acylphosphate + H2O | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Equus caballus | carboxylate + phosphate | - |
? | |
| ATP + H2O | muscle enzyme, very low activity | Equus caballus | ? | - |
? | |
| benzoyl phosphate + H2O | - |
Gallus gallus | benzoate + phosphate | - |
? | |
| benzoyl phosphate + H2O | - |
Homo sapiens | benzoate + phosphate | - |
? | |
| benzoyl phosphate + H2O | - |
Sus scrofa | benzoate + phosphate | - |
? | |
| benzoyl phosphate + H2O | - |
Bos taurus | benzoate + phosphate | - |
? | |
| benzoyl phosphate + H2O | - |
Oryctolagus cuniculus | benzoate + phosphate | - |
? | |
| benzoyl phosphate + H2O | - |
Equus caballus | benzoate + phosphate | - |
? | |
| carbamoyl phosphate + H2O | - |
Sus scrofa | carbamate + phosphate | - |
? | |
| carbamoyl phosphate + H2O | - |
Bos taurus | carbamate + phosphate | - |
? | |
| carbamoyl phosphate + H2O | - |
Equus caballus | carbamate + phosphate | - |
? | |
| carbamoyl phosphate + H2O | erythrocyte enzyme: not a substrate | Homo sapiens | carbamate + phosphate | - |
? | |
| diphosphate + H2O | muscle enzyme, very low activity | Equus caballus | 2 phosphate | - |
? | |
| diphosphate + H2O | erythrocyte enzyme, inorganic diphosphate: not a substrate | Homo sapiens | 2 phosphate | - |
? | |
| additional information | substrate specificity | Gallus gallus | ? | - |
? | |
| additional information | substrate specificity | Homo sapiens | ? | - |
? | |
| additional information | substrate specificity | Sus scrofa | ? | - |
? | |
| additional information | substrate specificity | Bos taurus | ? | - |
? | |
| additional information | substrate specificity | Oryctolagus cuniculus | ? | - |
? | |
| additional information | substrate specificity | Equus caballus | ? | - |
? | |
| additional information | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Gallus gallus | ? | - |
? | |
| additional information | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Homo sapiens | ? | - |
? | |
| additional information | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Sus scrofa | ? | - |
? | |
| additional information | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Bos taurus | ? | - |
? | |
| additional information | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Oryctolagus cuniculus | ? | - |
? | |
| additional information | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Equus caballus | ? | - |
? | |
| additional information | no activity with 3-phosphoglycerate, phosphocreatine, DL-3-glycerophosphate, fructose 1,6-diphosphate, glucose 6-phosphate, fructose 6-phosphate, phosphoenolpyruvate, ATP, ADP, AMP, cyclic-AMP, 6-phosphogluconate phosphoserine | Homo sapiens | ? | - |
? | |
| additional information | acts specifically by splitting off the carboxyl phosphate bond | Gallus gallus | ? | - |
? | |
| additional information | acts specifically by splitting off the carboxyl phosphate bond | Homo sapiens | ? | - |
? | |
| additional information | acts specifically by splitting off the carboxyl phosphate bond | Sus scrofa | ? | - |
? | |
| additional information | acts specifically by splitting off the carboxyl phosphate bond | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | acts specifically by splitting off the carboxyl phosphate bond | Bos taurus | ? | - |
? | |
| additional information | acts specifically by splitting off the carboxyl phosphate bond | Oryctolagus cuniculus | ? | - |
? | |
| additional information | acts specifically by splitting off the carboxyl phosphate bond | Equus caballus | ? | - |
? | |
| additional information | no activity with phosphoenolpyruvate and phosvitin | Equus caballus | ? | - |
? | |
| additional information | possible role in preventing the intracellular accumulation of 1,3-diphosphoglycerate by catalyzing the hydrolysis of this substrate to 3-phosphoglycerate | Gallus gallus | ? | - |
? | |
| additional information | possible role in preventing the intracellular accumulation of 1,3-diphosphoglycerate by catalyzing the hydrolysis of this substrate to 3-phosphoglycerate | Homo sapiens | ? | - |
? | |
| additional information | possible role in preventing the intracellular accumulation of 1,3-diphosphoglycerate by catalyzing the hydrolysis of this substrate to 3-phosphoglycerate | Sus scrofa | ? | - |
? | |
| additional information | possible role in preventing the intracellular accumulation of 1,3-diphosphoglycerate by catalyzing the hydrolysis of this substrate to 3-phosphoglycerate | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | possible role in preventing the intracellular accumulation of 1,3-diphosphoglycerate by catalyzing the hydrolysis of this substrate to 3-phosphoglycerate | Bos taurus | ? | - |
? | |
| additional information | possible role in preventing the intracellular accumulation of 1,3-diphosphoglycerate by catalyzing the hydrolysis of this substrate to 3-phosphoglycerate | Oryctolagus cuniculus | ? | - |
? | |
| additional information | possible role in preventing the intracellular accumulation of 1,3-diphosphoglycerate by catalyzing the hydrolysis of this substrate to 3-phosphoglycerate | Equus caballus | ? | - |
? | |
| additional information | enzyme could control acylation and carbamylation of proteins by regulation the levels of reactive acyl and carbamoyl phosphates | Equus caballus | ? | - |
? | |
| additional information | possible physiological role for acylphosphatase activity may be regulation of metabolic pathways involving 1,3-diphosphoglycerate and carbamoyl phosphate, e.g. glycolytic pathway and pyrimidine biosynthesis, by its ability to hydrolyze these substrates | Equus caballus | ? | - |
? | |
| p-nitrobenzoyl phosphate + H2O | - |
Homo sapiens | p-nitrobenzoate + phosphate | - |
? | |
| p-nitrobenzoyl phosphate + H2O | - |
Equus caballus | p-nitrobenzoate + phosphate | - |
? | |
| p-nitrophenyl phosphate + H2O | erythrocyte enzyme, not a substrate | Homo sapiens | p-nitrophenol + phosphate | - |
? | |
| p-nitrophenyl phosphate + H2O | muscle enzyme, very low activity at pH 5.3, no activity at pH 10.4 | Equus caballus | p-nitrophenol + phosphate | - |
? | |
| phosphocreatine + H2O | muscle enzyme, very low activity | Equus caballus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Gallus gallus |
| 25 | - |
assay at | Homo sapiens |
| 25 | - |
assay at | Sus scrofa |
| 25 | - |
assay at | Saccharomyces cerevisiae |
| 25 | - |
assay at | Bos taurus |
| 25 | - |
assay at | Oryctolagus cuniculus |
| 25 | - |
assay at | Equus caballus |
| 27 | - |
assay at | Sus scrofa |
| 27 | - |
assay at | Bos taurus |
| 37 | - |
erythrocyte, assay at | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Gallus gallus |
| additional information | - |
enzyme is thermostable | Bos taurus |
| additional information | - |
enzyme is thermostable | Equus caballus |
| additional information | - |
muscle and brain enzymes: stable at high temperatures | Homo sapiens |
| 70 | - |
erythrocyte enzyme, 5 min: 40% loss of activity | Homo sapiens |
| 85 | - |
heart enzyme, pH 2.0, 5 min, 95% activity retained | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Gallus gallus |
| additional information | - |
muscle, pI: 11.4 | Homo sapiens |
| additional information | - |
muscle, pI: 11.4 | Equus caballus |
| additional information | - |
heart, pI: 7.25-7.3 | Sus scrofa |
| additional information | - |
erythrocyte enzyme is a basic protein | Homo sapiens |
| 5 | - |
- |
Gallus gallus |
| 5 | - |
- |
Sus scrofa |
| 5 | - |
erythrocyte enzyme, substrate: acetyl phosphate | Homo sapiens |
| 5.3 | - |
assay at | Gallus gallus |
| 5.3 | - |
assay at | Homo sapiens |
| 5.3 | - |
assay at | Sus scrofa |
| 5.3 | - |
assay at | Saccharomyces cerevisiae |
| 5.3 | - |
assay at | Bos taurus |
| 5.3 | - |
assay at | Oryctolagus cuniculus |
| 5.3 | - |
assay at | Equus caballus |
| 5.3 | - |
muscle and liver | Equus caballus |
| 5.4 | - |
- |
Oryctolagus cuniculus |
| 5.4 | - |
erythrocyte, assay at | Homo sapiens |
| 5.4 | 5.6 | heart enzyme, substrate: acetyl phosphate | Sus scrofa |
| 6 | - |
assay at | Sus scrofa |
| 6 | - |
assay at | Bos taurus |
| 7.4 | 7.6 | brain, substrate: acetyl phosphate | Bos taurus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Gallus gallus |
| additional information | - |
- |
Homo sapiens |
| additional information | - |
- |
Oryctolagus cuniculus |
| additional information | - |
brain enzyme is acid stable | Bos taurus |
| additional information | - |
liver enzyme is acid stable | Equus caballus |
| 1.5 | 2 | heart enzyme, 100% activity retained | Sus scrofa |
| 9 | - |
- |
Oryctolagus cuniculus |
| 9 | - |
heart enzyme, 92% activity retained | Sus scrofa |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.2 | - |
fructose 1,6-diphosphate | noncompetitive inhibition | Equus caballus | |
| 3.5 | - |
methyl phosphate | moderate competitive inhibition | Equus caballus | |
| 6.9 | - |
Carbamoyl phosphate | erythrocyte enzyme, competitive inhibition | Homo sapiens | |
| 11 | - |
benzyl phosphate | moderate competitive inhibition | Equus caballus | |
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Release 2023.1 (January 2023)
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