Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.172 | - |
5-Methyl-dCTP | pH 9.0, 37°C | Escherichia coli | |
0.551 | - |
CTP | pH 9.0, 37°C | Escherichia coli | |
0.767 | - |
dCTP | pH 9.0, 37°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | most effective cation, with optimal activity at 5 mM Mg2+ | Escherichia coli | |
Mn2+ | about 10% of the activity with Mg2+ | Escherichia coli | |
additional information | absolute requirement for a divalnt cation. No activity in presence of Zn2+, Co2+, Ca2+ | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P77788 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
193 | - |
substrate 5-methyl-dCTP, pH 9.0, 37°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-methyl-dCTP + H2O | - |
Escherichia coli | 5-methyl-dCMP + diphosphate | - |
? | |
arabinosyl-CTP + H2O | 3% of the activity with 5-methyl-dCTP | Escherichia coli | arabinosyl-CMP + diphosphate | - |
? | |
CDP + H2O | 2% of the activity with 5-methyl-dCTP | Escherichia coli | cytidine + diphosphate | - |
? | |
CTP + H2O | 48% of the activity with 5-methyl-dCTP | Escherichia coli | CMP + diphosphate | - |
? | |
dCTP + H2O | 23% of the activity with 5-methyl-dCTP | Escherichia coli | dCMP + diphosphate | - |
? | |
dTTP + H2O | 2% of the activity with 5-methyl-dCTP | Escherichia coli | dTMP + diphosphate | - |
? | |
dUTP + H2O | 1% of the activity with 5-methyl-dCTP | Escherichia coli | dUMP + diphosphate | - |
? | |
additional information | enzyme is highly specific for pyrimidine (deoxy)nucleoside triphosphates. Like other Nudix hydrolases, the enzyme cleaves its substrates to produce a nucleoside monophosphate and diphosphate | Escherichia coli | ? | - |
? | |
additional information | no substrates: CMP, dCMP, dATP, ATP, dGTP, GTP, dUTP, CDP-ethanolamine, CDP-choline, CDP-glucose, CDP-glycerol | Escherichia coli | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14.3 | - |
dCTP | pH 9.0, 37°C | Escherichia coli | |
25.8 | - |
CTP | pH 9.0, 37°C | Escherichia coli | |
51.7 | - |
5-Methyl-dCTP | pH 9.0, 37°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | - |
Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18 | - |
dCTP | pH 9.0, 37°C | Escherichia coli | |
47 | - |
CTP | pH 9.0, 37°C | Escherichia coli | |
301 | - |
5-Methyl-dCTP | pH 9.0, 37°C | Escherichia coli |