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Literature summary for 3.6.1.65 extracted from

  • Fujikawa, K.; Kasai, H.
    The oxidized pyrimidine ribonucleotide, 5-hydroxy-CTP, is hydrolyzed efficiently by the Escherichia coli recombinant Orf135 protein (2002), DNA Repair, 1, 571-576.
    View publication on PubMed
Search for more literature for 3.6.1.65

Cloned(Commentary)

Cloned (Comment) Organism
expression as glutathione S-transferase fusion protein Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0557
-
 5-hydroxy-CTP pH 8.5, 30°C Escherichia coli
0.428
-
 CTP pH 8.5, 30°C Escherichia coli
1.72
-
 dCTP pH 8.5, 30°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, activity constant 1.2 mM Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P77788
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-hydroxy-CTP + H2O
-
 Escherichia coli 5-hydroxy-CMP + diphosphate
-
 ?
5-hydroxy-dCTP + H2O
-
 Escherichia coli 5-hydroxy-dCMP + diphosphate 4.3% of the activity with 5-hydroxy-CTP ?
CTP + H2O
-
 Escherichia coli CMP + diphosphate
-
 ?
dCTP + H2O
-
 Escherichia coli dCMP + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
nudG
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.28
-
 CTP pH 8.5, 30°C Escherichia coli
17.4
-
 dCTP pH 8.5, 30°C Escherichia coli
35.1
-
 5-hydroxy-CTP pH 8.5, 30°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
10.1
-
 dCTP pH 8.5, 30°C Escherichia coli
21.7
-
 CTP pH 8.5, 30°C Escherichia coli
630
-
 5-hydroxy-CTP pH 8.5, 30°C Escherichia coli