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show all sequences of 3.6.1.64

Structural basis for the specificity of human NUDT16 and its regulation by inosine monophosphate

Tresaugues, L.; Lundbaeck, T.; Welin, M.; Flodin, S.; Nyman, T.; Silvander, C.; Graeslund, S.; Nordlund, P.; PLoS ONE 10, e0131507 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Homo sapiens
Crystallization (Commentary)
Crystallization
Organism
mutant A22V,structure forms an alpha/beta/alpha sandwich which is constituted by two beta-sheets, one being composed of two parallel beta-strands lined by two anti-parallel beta-strands and the second one by three anti-parallel beta-strands. The protein is decorated with five additional motifs being part of the hydrophobic core or serving roles to stabilize the dimer
Homo sapiens
Engineering
Amino acid exchange
Commentary
Organism
A22V
crystallization data
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
ADP
-
Homo sapiens
GDP
-
Homo sapiens
IDP
-
Homo sapiens
IMP
product inhibition
Homo sapiens
ITP
-
Homo sapiens
XDP
-
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0062
-
XDP
pH 7.5, 37°C
Homo sapiens
0.0082
-
ITP
pH 7.5, 37°C
Homo sapiens
0.285
-
ADP
pH 7.5, 37°C
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
Q96DE0
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP + H2O
-
735030
Homo sapiens
AMP + phosphate
-
-
-
?
ITP + H2O
-
735030
Homo sapiens
IDP + phosphate
-
-
-
?
additional information
rate of the catalytic cycle is primarily regulated by the product-release step
735030
Homo sapiens
?
-
-
-
-
XDP + H2O
-
735030
Homo sapiens
XMP + phosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
crystallization data
Homo sapiens
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.006
-
XDP
pH 7.5, 37°C
Homo sapiens
0.013
-
ITP
pH 7.5, 37°C
Homo sapiens
0.096
-
ADP
pH 7.5, 37°C
Homo sapiens
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0098
-
IMP
substrate IDP, pH 7.5, 37°C
Homo sapiens
0.0163
-
IMP
substrate IDP, pH 7.5, 37°C
Homo sapiens
0.0213
-
IMP
substrate GDP, pH 7.5, 37°C
Homo sapiens
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.024
-
substrate IDP, pH 7.5, 37°C
Homo sapiens
IMP
0.795
-
substrate GDP, pH 7.5, 37°C
Homo sapiens
IMP
3.174
-
substrate IDP, pH 7.5, 37°C
Homo sapiens
IMP
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Homo sapiens
Crystallization (Commentary) (protein specific)
Crystallization
Organism
mutant A22V,structure forms an alpha/beta/alpha sandwich which is constituted by two beta-sheets, one being composed of two parallel beta-strands lined by two anti-parallel beta-strands and the second one by three anti-parallel beta-strands. The protein is decorated with five additional motifs being part of the hydrophobic core or serving roles to stabilize the dimer
Homo sapiens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
A22V
crystallization data
Homo sapiens
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.024
-
substrate IDP, pH 7.5, 37°C
Homo sapiens
IMP
0.795
-
substrate GDP, pH 7.5, 37°C
Homo sapiens
IMP
3.174
-
substrate IDP, pH 7.5, 37°C
Homo sapiens
IMP
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ADP
-
Homo sapiens
GDP
-
Homo sapiens
IDP
-
Homo sapiens
IMP
product inhibition
Homo sapiens
ITP
-
Homo sapiens
XDP
-
Homo sapiens
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0098
-
IMP
substrate IDP, pH 7.5, 37°C
Homo sapiens
0.0163
-
IMP
substrate IDP, pH 7.5, 37°C
Homo sapiens
0.0213
-
IMP
substrate GDP, pH 7.5, 37°C
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0062
-
XDP
pH 7.5, 37°C
Homo sapiens
0.0082
-
ITP
pH 7.5, 37°C
Homo sapiens
0.285
-
ADP
pH 7.5, 37°C
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP + H2O
-
735030
Homo sapiens
AMP + phosphate
-
-
-
?
ITP + H2O
-
735030
Homo sapiens
IDP + phosphate
-
-
-
?
additional information
rate of the catalytic cycle is primarily regulated by the product-release step
735030
Homo sapiens
?
-
-
-
-
XDP + H2O
-
735030
Homo sapiens
XMP + phosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
crystallization data
Homo sapiens
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.006
-
XDP
pH 7.5, 37°C
Homo sapiens
0.013
-
ITP
pH 7.5, 37°C
Homo sapiens
0.096
-
ADP
pH 7.5, 37°C
Homo sapiens
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.52
-
ADP
pH 7.5, 37°C
Homo sapiens
0.99
-
XDP
pH 7.5, 37°C
Homo sapiens
1.55
-
ITP
pH 7.5, 37°C
Homo sapiens
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.52
-
ADP
pH 7.5, 37°C
Homo sapiens
0.99
-
XDP
pH 7.5, 37°C
Homo sapiens
1.55
-
ITP
pH 7.5, 37°C
Homo sapiens
Other publictions for EC 3.6.1.64
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735030
Tresaugues
Structural basis for the speci ...
Homo sapiens
PLoS ONE
10
e0131507
2015
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4
1
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3
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3
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3
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1
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1
1
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3
6
3
3
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4
1
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3
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3
3
720583
Iyama
NUDT16 is a (deoxy)inosine dip ...
Homo sapiens, Mus musculus
Nucleic Acids Res.
38
4834-4843
2010
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1
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7
4
3
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4
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2
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3
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9
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1
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7
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1
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7
4
3
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3
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9
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1
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7
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1
1
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7
7